Dibohemamines A–C (5–7), three novel dimeric bohemamine analogs dimerized through a methylene group, were isolated from a marine-derived Streptomyces spinoverrucosus. The structures determined by spectroscopic analysis were confirmed through the semi-synthetic derivatization of monomeric bohemamines and formaldehyde. These reactions, which could occur under mild conditions, together with the detection of formaldehyde in the culture, revealed that this dimerization is a non-enzymatic process. In addition to the unique dimerization of the dibohemamines, dibohemamines B and C were found to have nM cytotoxicity against the non-small cell lung cancer cell line A549. In view of the potent cytotoxicity of compounds 6 and 7, a small library of bohemamine analogs w as generated for biological evaluation by utilizing a series of aryl and alkyl aldehydes.
Inducamides A–C (1–3),
three new chlorinated alkaloids featuring an amide skeleton generated
by a tryptophan fragment and a 6-methylsalicylic acid unit, were isolated
from a chemically induced mutant strain of Streptomyces sp. with the inducamides only being produced in the mutant strain.
Their structures, including stereochemistry, were determined by spectroscopic
analysis, Marfey’s method, and CD spectroscopy.
Two new 1,3-oxazin-6-one derivatives
(1 and 2) and six new bohemamine-type pyrrolizidine
alkaloids (3–8) were isolated from
the marine-derived Streptomyces spinoverrucosus strain
SNB-048. Their structures
including the absolute configurations were fully elucidated on the
basis of spectroscopic analysis, ECD spectra, quantum chemical calculations,
and chemical methods. Compounds 1 and 2 possess
a γ-lactam moiety and a 1,3-oxazin-6-one system.
The systematic screening of asymptomatic and pre-symptomatic individuals is a powerful tool for controlling community transmission of infectious disease on college campuses. Faced with a paucity of testing in the beginning of the COVID-19 pandemic, many universities developed molecular diagnostic laboratories focused on SARS-CoV-2 diagnostic testing on campus and in their broader communities. We established the UC Santa Cruz Molecular Diagnostic Lab in early April 2020 and began testing clinical samples just five weeks later. Using a clinically-validated laboratory developed test (LDT) that avoided supply chain constraints, an automated sample pooling and processing workflow, and a custom laboratory information management system (LIMS), we expanded testing from a handful of clinical samples per day to thousands per day with the testing capacity to screen our entire campus population twice per week. In this report we describe the technical, logistical, and regulatory processes that enabled our pop-up lab to scale testing and reporting capacity to thousands of tests per day.
Three new cyclohexene amine derivatives, daryamides D-F (1-3), a new arylamine derivative, carpatamide D (4), and a new ornithine lactamization derivative, ornilactam A (5), were isolated from the marine-derived Streptomyces strain SNE-011. Their structures, including absolute configurations, were elucidated on the basis of spectroscopic analysis and chemical methods. The carpatamide skeleton could be considered as the biosynthetic precursor of the daryamides.
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