No abstract

No abstract

For the first time, a non-catalytic and myotoxic Lys49-PLA 2 (BthTX-I from Bothrops jararacussu venom) has been crystallized with BPB inhibitor. X-ray diffraction data were collected and electron-density calculations showed that the ligand is bound to the His48 residue. BthTX-I with His48 chemically modified by BPB shows strongly reduced myotoxic and cytotoxic activities. This suggests a biological correlation between the modification of His48, which is associated with catalytic activity of PLA 2 s, and other toxicological activities of Lys49-PLA 2 s.

Plant-antivenom is a computational Websystem about medicinal plants with anti-venom properties. The system consists of a database of these plants, including scientific publications on this subject and amino acid sequences of active principles from venomous animals. The system relates these data allowing their integration through different search applications. For the development of the system, the first surveys were conducted in scientific literature, allowing the creation of a publication database in a library for reading and user interaction. Then, classes of categories were created, allowing the use of tags and the organization of content. This database on medicinal plants has information such as family, species, isolated compounds, activity, inhibited animal venoms, among others. Provision is made for submission of new information by registered users, by the use of wiki tools. Content submitted is released in accordance to permission rules defined by the system. The database on biological venom protein amino acid sequences was structured from the essential information from National Center for Biotechnology Information (NCBI). Plant-antivenom's interface is simple, contributing to a fast and functional access to the system and the integration of different data registered on it. Plant-antivenom system is available on the Internet at

Anemopaegma arvense (Vell.) Stellfeld, Bignoniaceae, is a native species of the Brazilian savanna (Cerrado) and is commonly known as catuaba among the local farmers. Seeds of three varieties were collected in different localities and submitted to germination and storage studies in attempting to domesticate this species as a medicinal crop for small farmers located in Brazilian Cerrado. Germination tests revealed that catuaba seeds presented a dormancy period of 6 weeks, and 63% of the seedlings have emerged after 12 weeks of the planting time. Storing catuaba seeds at low temperatures (-20 and -196°C) has not affected emergence and survival. These findings suggested that A. arvense seeds have an orthodox behavior resisting well to dehydration and low temperature storage. Three catuaba varieties were characterized morphologically and chemically. The presence of triterpenes such as oleanolic acid and betulinic acid were identified and quantified in these varieties. Previous report has shown that these compounds have promising anticancer activities and herein the results point that the aerial parts yielded more triterpenes than the roots. The combination of higher capacity and preferential accumulation of triterpenes in the aerial parts of catuaba makes this plant a potential candidate for agricultural production or in situ sustainable harvests as a promising alternative to the destructive collection of the natural population.