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A procedure has been developed for high frequency adventitious shoot regeneration from lzypocotyls, cotyledon, stem and petiole explants of cicer mil kvetch. All explants isolated from in vitro seedlings were cultured on Murashige and Skoog ( MS) media supplemented with various concentrations of 6-benz_vlaminopurine (BA) and a-naphthaleneacetic acid (NAA) to induce adventitious shoot regeneration. Hypocotyl explants appeared to have better regeneration capacity than stem, cotyledon and petiole explants in most of the media tested. The highest frequency of shoot regeneration was achieved from hypocotyl segments through an initial callus growth on MS medium containing 10 J.IM BA and 0.1 J1M NAA. Regenerated shoots were excised and rooted in half-strength MS medium supplemented with 5.4 J.IM NAA. Rooted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.

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Transformation of fenugreek (Trigonella foenumgraecum) was carried out with A281 oncogenic strain of Agrobacterium tumefaciens using root, cotyledon and hypocotyl explants excised from 1-week-old seedlings, which showed that the plant was highly susceptible to transformation. Tumors (calli) were selected on 50 mg dm -3 kanamycin. They were analyzed for β-glucuronidase (GUS) expression. Presence of uidA (gus) gene, was confirmed by polymerase chain reaction (PCR) amplification.

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