<i>In Vitro</i>Bulblet Induction from Bulb Scales of Endangered Ornamental Plant<i>Muscari Azureum</i>

Uranbey, Serkan; İpek, Ahmet; Çalışkan, Mehmet Emin; Dündar, Ekrem; Cocu, Sati; Başalma, Dilek; Güneylioğlu, Hümeyra

doi:10.2478/v10133-010-0024-4

Cited by 16 publications

(9 citation statements)

References 14 publications

(11 reference statements)

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“…Increasing TDZ concentration decreased bulblet production. Similar to these findings, TDZ did not effectively promote bulblet regeneration in M. aucheri and M. azureum (Uranbey, 2010a(Uranbey, , 2010bUranbey et al, 2010). …”

Section: Discussionsupporting

confidence: 77%

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Uzun¹,

Parmaksız²,

Uranbey³

et al. 2014

Turk J Biol

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An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of α-naphthalene acetic acid (NAA), picloram, dicamba, 6-benzylaminopurine (BAP), and thidiazuron (TDZ). The best bulblet regeneration (59 bulblets per explant) was achieved in Murashige and Skoog (MS) medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.

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Section: Discussionsupporting

confidence: 77%

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Uzun¹,

Parmaksız²,

Uranbey³

et al. 2014

Turk J Biol

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“…The results of the study suggest that the indvidual regeneration on TDZ-NAA-containing MS medium was strongly influenced by the concentrations and combinations of plant growth regulators. The results are in agreement with Uranbey (2010 a, b), Uranbey et al (2011), Ozel et al (2007, 2009, 2015, Vaziri et al (2014) and Uzun et al (2014), who reported the propagation of other Turkish Muscari species using different cytokinins and auxin concentrations.…”

Section: Bulblet Regeneration Behavioursupporting

confidence: 90%

“…All (100%) bulbs induced under in vitro conditions were easily acclimatised. However Uranbey et al (2011) used 5-10 mm bulblets of M. azureum and acclimatised them on compost with a survival rate of 3%. The possible reason could be different type of materials used for rooting.…”

Section: Bulblet Diametermentioning

confidence: 99%

“…This experiment induced rooting under controlled in vitro conditions. Whereas, Uranbey et al (2011) used compost instead to root the Muscari bulbs. It seems as if the Nitrogen rich compost had negative effect on the bulbs that most often results in burning of the plant material.…”

Section: Bulblet Diametermentioning

confidence: 99%

“…On the other hand, Uzun et al (2014) induced 100% acclimatization of Muscari muscarimi in peat moss, vermiculite and perlite (1:1:1) mixture. Uranbey et al (2011) used 5-10 mm bulblets of M. azureum and acclimatized them on compost with a survival rate of 3%. The possible reason for this observation could be the difference in genotypes, cultural conditions and type of composts that were used in two experiments during acclimatisation.…”

Section: Acclimizationmentioning

confidence: 99%

See 2 more Smart Citations

Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Özel

Ünal

2016

Turkish JAF Sci.Tech.

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Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant.

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Meta-topolin induced morphometric and structurally stable bulblets in Malabar River Lily (Amaryllidaceae)

Manokari

Priyadharshini

Cokulraj

et al. 2021

Plant Cell Tiss Organ Cult

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In VitroBulblet Induction from Bulb Scales of Endangered Ornamental PlantMuscari Azureum

Cited by 16 publications

References 14 publications

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Meta-topolin induced morphometric and structurally stable bulblets in Malabar River Lily (Amaryllidaceae)

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