Aim:The present study was conducted to ascertain the prevalence of ketosis in dairy cows in dairy herds, milksheds, and mixed population of milk cows selected randomly in milkshed areas of Odisha state, India.Materials and Methods:The investigation was conducted in 280 private dairy herds with variable herd size of 10-15 cows comprising crossbred Jersey cows (CBJ), crossbred Holstein Friesian (CHF) cows, and indigenous local breeds. The analysis of urine (Rothera’s test), milk (Ross test), and blood samples of 2760 test cows were conducted through qualitative assessment by Rothera’s test and Ross test, respectively, for the presence of ketone bodies to screen the ketotic animals. Cut-points have been decided based on β-hydroxybutyric acid level (≥1.2-1.4 mmol/L) in milk.Results:We noted positive cases of ketosis with a prevalence rate of 36.7% (1014/2760) entailing 27.2% in clinical ketosis and 9.6% in subclinical ketosis. The breed wise incident rate was recorded to be the highest (38.0%) in CBJs. The age-wise prevalence rate was found to be the highest (40.8%) in the age group of 5.5-6.5 years. The season wise prevalence rate in 5th calver was recorded to be the highest (38.6%) in summer season as compared to other seasons. The prevalence of ketosis was observed to be the highest at 56.7% on the first stage of lactation at the 1st month after 2 weeks. The incidence rates for clinical and subclinical ketosis were found to be 25.2%; 12.2%, 26.6%; 11.2% and 30.3%; 2.9% in CBJ, CHF and indigenous cows, respectively. The breed wise overall prevalence rate was recorded to be 38.0% in CBJ, 37.8% in CHF, and 33.2% in indigenous cows.Conclusion:Ketosis and subclinical ketosis is highly prevalent metabolic disorder and has severe effect on the production status of affected animal and needs to be prevented, rather than treated, by maintaining cows in good and healthy conditions. We have attempted to give great attention for diagnosis, management, and control of this disease during risk stage to prevent economic loss sustained by the dairy farmers of Eastern India.

Background and Aim: Periodontitis is the most prevalent inflammatory dental disease caused by a lack of oral hygiene measures in domestic animals. The periodontal disease complex arises as a result of bacterial biofilm deposition termed as plaque on the tooth surface. Lack of cleaning measures either mechanical or chemical credit for the condition. The present study was conducted to screen the animals for the presence of plaque deposition, gingivitis, along with various control measures for the same. Materials and Methods: Thirty-two dogs of different age groups were evaluated for the presence of plaque and gingivitis by scoring method to estimate the extent of severity. Scaling of the tooth surface was done by ultrasonic scaling machine to remove the plaques, and the animals were divided into four treatment groups to study the effects of dental hygiene chew and chlorhexidine for control of plaque. Results: Present study revealed 71.87% and 34.37% of the screened animals were having plaque deposition and varied degrees of gingivitis respectively. A positive coefficient of correlation (r) of 0.89 (p<0.05) between advancing age and plaque deposition and 0.85 (p<0.05) between age and level of gingivitis was obtained. Two groups receiving dental chew and 0.2% w/v chlorhexidine showed lower plaque deposits, and the fourth treatment group receiving both dental chew and chlorhexidine showed 100% animals remained free from fresh plaque deposits. Conclusion: The present study showed a strong positive relationship between age and plaque deposition and gingivitis. The study also showed that oral hygiene measures such as use of dental hygiene chew and chlorhexidine application can reduce plaque deposition and periodontitis in domesticated canines.

During the years 2010–2018, avipoxvirus (APV) outbreaks were observed in the domestic chickens and pigeons present in the eastern Indian state of Odisha. Based on typical pox lesions, followed by molecular techniques, the overall morbidity was found to be 18%–19.23% and 16.92%–23% in chickens and pigeons, respectively. The cutaneous forms of the disease were observed with varied rates of mortality, being 47.36%–52.77% in chickens and 39.13%–92% in pigeons. PCR amplification targeting the viral P4b core protein‐coding gene and the DNA polymerase gene confirmed the presence of APV strains in 10 birds. Subsequent phylogenetic analysis of these two genes confirmed that the circulating strains were members of APV clade A. The subclade analysis revealed the introduction of A1 and A3 subclades in Indian chickens and pigeons, respectively. This study is the first molecular record of APVs circulating in eastern Indian birds (Odisha) and involves the first use of the polymerase gene to reveal the circulating clades of Indian APVs.

Lumpy skin disease virus (LSDV) is the causative agent of lumpy skin disease (LSD) which is a member of Capripoxvirus. It is an economically critical transboundary disease affecting cattle. This study records the first LSD incidence in cattle of Ganjam district and analyses data from LSD outbreak in August 2020 on epidemiological and genetic characterization. Out of 452 animals clinically examined (59 farms), 63 animals were clinically affected with LSD, with a total morbidity rate of 13.93%. The morbidity rates in the villages (ten villages) varied from 5.55 to 21.62%. The multivariable logistic regression showed that grazing of animals (P=0.023; OR: 1.90; 95% CI: 1.09-3.32), and lactation and pregnancy status of animals (P=0.007; OR: 2.86; 95% CI: 1.32-6.17) were the potential risk factors for the occurrence of lumpy skin disease. Out of 53 clinically suspected animals collected from Ganjam district of Odisha, 18 samples (33.96%) were found positive by PCR for both P32 and F genes of capripox virus. Phylogenetic analysis of P32 gene of LSD (MW147486) showed 100% similarity with other isolates from India, Bangladesh, Egypt and Saudi Arabia. Additionally, phylogenetic analysis of F gene of LSD (MW147485) revealed a similarity of 97.99 %, 97.36%, and 96.60% with, Odisha India (MT074110), Beni Suif Egypt (MN694826) and Marsa Matrouh Egypt (MN699855), respectively.

Aim:The study was conducted to determine the serum levels of certain hormones in post-partum anestrus cows following treatment with controlled internal drug release (CIDR) and Ovsynch protocol.Materials and Methods:A total of 30 postpartum anestrus cows were divided into three equal groups after thorough gynecoclinical examination. The Group 1 animals received an intravaginal progesterone device on day 0 and 2 ml of prostaglandin F2α (PGF2α) on day of CIDR removal (7th day), Group 2 cows were treated with ovsynch protocol (gonadotropin-releasing hormone [GnRH]-PGF2α-GnRH) on day 0, 7 and 9, respectively, and Group 3 cows were supplemented with mineral mixture and treated as control. The serum estrogen, progesterone, triiodothyronine, and thyroxine concentration were estimated using enzyme-linked immunosorbent assay kit and absorbance was read at 450 nm with Perkin Elmer Wallac 1420 Microplate Reader.Results:There was a significant increase in progesterone level in Group 1 after withdrawal of CIDR as compared to other two groups. However, the estrogen assay revealed a greater concentration in Group 2 against Group 1 on day 7 of sampling. However, there was no significant difference for serum triiodothyronine (T3) and thyroxine (T4) irrespective of treatment protocols and days of sampling.Conclusion:Treatment with CIDR based progesterone therapy and drug combinations may affect the reproductive hormonal balance like estrogen and progesterone, which is inevitable for successful return to cyclicity and subsequent fertilization and conception. However, as far as serum T3 and T4 concentration concerned it may not give an astounding result.