Our study established the histopathlogical effect of different dosages malathion on the third larval instar of Chrysomya megacephala. The concentration of malathion increased gradually during the larval stages reaching the maximum concentration in the third instar larvae.The midgut of third larval instar was studied using transmission electron microscope. The most significant alterations were increasing damage to the larvae midgut including columnar prominent ,fat vacuoles, atrophied microvilli. These results indicate that malathion retard larval development.
The Journal of Medical Entomology and Parasitology is one of the series issued quarterly by the Egyptian Academic Journal of Biological Sciences. It is an important specialist journal covering the latest advances in that subject. It publishes original research and review papers on all aspects of basic and applied medical entomology, parasitology and host-parasite relationships, including the latest discoveries in parasite biochemistry, molecular biology, genetics, ecology and epidemiology in the content of the biological, medical entomology and veterinary sciences. In addition to that, the journal promotes research on the impact of living organisms on their environment with emphasis on subjects such a resource, depletion, pollution, biodiversity, ecosystem…..etc. www.eajbs.eg.netProvided for non-commercial research and education use. Not for reproduction, distribution or commercial use.This study investigated the potential effects of Malathion in rabbit carcasses on the developmental of Chrysomya megacephala larvea, an important forensic species, and their possible implications for the calculate the postmortm interval ( PMI) .Three domestic rabbits Oryctolagus cuniculus were used in each experiment. Two rabbits were administered orally with 513 mg/kg (R1), 1026 mg/kg (R2) of Malathion, The third one was fed with distilled water (R0).Chrysomya megacephala larvae were allowed to grow on the liver (L), muscles (M) and all carcasses of rabbit. Malathion was detected in all collected rabbit tissues that received different dosages of Malathion (R1 and R2) but not detected in any of the controls (R0).The highest Malathion concentration was detected in lungs followed by muscle tissue and fats of R2 (35.7 mg/kg, 29.75 mg/kg, and 22.31mg/kg, respectively). All third larval instars and pupae of C. megacephala were positive for malathion (R1 and R2), while malathion was not detected in all samples from the control colony (R0).Strong correlations were found between administered dosage and tissue concentrations. Malathion concentrations were higher in the third larval instars sampled for the concentration (R2) than those from muscle tissues of (R2) which followed by those from L2 colonies. Detected Malathion concentrations in the third larval instars and pupae of C. megacephala were significantly lower than those detected in the rabbit tissues except fats and heart from postmortem interval rabbit tissues treated with (R1). Moreover, the highest levels of Malathion were noticed in the pupae emerged from (R2) colony. Whereas, the lowest levels were detected in the pupae emerged from M1 colony.
Legionella spp. is transmitted from water to humans by aerosol-generating devices, including cooling towers (CTs). There have not been published reports about Legionella in these systems in Qatar. Ten CTs in Qatar University were sampled on a monthly basis. Bacteria were recovered from 90 water samples by filtration and concentration. Legionella DNA copy number (CN) was assessed by quantitative RT-PCR. Legionella DNA was detected in 100% of the samples. The bacterial counts ranged from 0.006 to 199.56 CFU/mL, and critical counts were found in 51 (56.7 %) samples. Moreover, 7 (7.8%) samples showed a count of more than 100 CFU/mL. The highest counts were found in the months of May and June. These results suggest that this organism is found in high number in tested CTs, presenting a potential health risk to the local population.https://doi.org/10.26719/2017.23.10.703Première étude au Qatar pour mettre en évidence la forte présence de légionelles dans les tours de refroidissement RÉSUMÉ Les Legionella spp se transmettent de l'eau à l'homme par les dispositifs générateurs d'aérosols, notamment les tours de refroidissement. Aucun rapport n'a été publié sur la présence de légionelles dans ces systèmes au Qatar. Des prélèvements mensuels ont été effectués dans dix tours de refroidissement de l'Université du Qatar. Des bactéries ont été retrouvées dans 90 échantillons d'eau par filtration et concentration. Le nombre de copies de l'ADN des Legionella a été évalué par PCR quantitative en temps réel. L'ADN des Legionella a été détecté dans 100 % des échantillons. La numération bactérienne était comprise entre 0,006 et 199,56 CFU/mL et des numérations critiques ont été constatées dans 51 échantillons (56,7 %). En outre, 7 échantillons (7,8 %) présentaient une numération supérieure à 100 CFU/mL. Les numérations les plus élevées ont été relevées aux mois de mai et de juin. Ces résultats semblent indiquer que cet organisme est présent en grand nombre dans les tours de refroidissement ayant fait l'objet de prélèvements, ce qui constitue un risque sanitaire potentiel pour la population locale.
This study investigated the effects of three temperature regimes representing several specific development conditions of this blowfly: (25.7, 28.4and 30.6 o C) and tissue types. Furthermore, Chrysomya megacephala colonies were reared on three different food sources (all rabbit carcasses, Liver and muscles) on the development of C.megacephala as important forensic fly, and their possible implications to calculate the postmortem interval (PMI). The results showed that the mean durations period from egg to adult eclosion of C. megacephala when reared on control rabbit at 25.7, 28.4 and 30.6 o C were 303.5, 217 and194.5 hrs, respectively. In addition, the mean duration period from egg to adult eclosion when the larvae reared on liver tissue at 25.7, 28.4and 30.6oC were 275.5, 205.5 and 188.5 hrs, respectively. Moreover, The Larvae of C.megacephala reared on muscle tissues from rabbit carcasses, the mean duration from egg to adult eclosion at 25.7 o C 28.4 o C, and 30.6 o C were 293, 214.5 and 192.5 hrs, respectively. From the previous results, it is obvious that the mean duration in larvae reared on control rabbit carcasses was longer than those fed on muscle which longer than those reared on the liver at all temperature regimes
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