Ultrastructure of the Midgut of theThird Larval Instar of Chrysomya megacephala (Diptera:Calliphoridae) fed on malathion treated diet

Abstract

Our study established the histopathlogical effect of different dosages malathion on the third larval instar of Chrysomya megacephala. The concentration of malathion increased gradually during the larval stages reaching the maximum concentration in the third instar larvae.The midgut of third larval instar was studied using transmission electron microscope. The most significant alterations were increasing damage to the larvae midgut including columnar prominent ,fat vacuoles, atrophied microvilli. These results indicate that malathion retard larval development.

Imidacloprid‐induced mortality, histopathology and biochemical impairments in the larvae of oriental latrine fly (Chrysomya megacephala)

“…The observed ultrastructural changes were more severe in larvae exposed to the insecticide for 48‐h compared with those exposed for 24‐h. The current study's findings are consistent with those of Bakr et al (2012), who observed that malathion caused an ultrastructural effect in the midgut cells of C. megacephala , evidenced by degenerated mitochondria and endoplasmic reticulum, and shrivelled microvilli.…”

Effects of Tramadol and Cypermethrin on the Histology of Sarcophaga ruficornis Larvae and Their Concentrations in Postmortem Rabbit Tissues and Their Post-Feeding Larvae

“…This damage included the notable enlargement and malformed shape in cubical epithelial cells, with a detachment of epithelial cells from the basement membrane in the lumen. Similar results were recorded in Spodoptera frugiperda treated with Azadirachta indica (Roel et al, 2010), in the larvae of Chrysomya megacephala treated with malathion (Bakr et al, 2012), in Chironomus calligraphus larvae fed with cypermethrin (Lavarías et al, 2017), and in Spodoptera litura exposed to leaf extract of Mirabilis jalapa (Suryani et al, 2020). Also, the treatment caused other damage in the midgut, with some epithelial cells showing symptoms of shrinking and others of swelling; the appearance of vacuoles in epithelial cells was accompanied by the destruction of the gut wall and epithelial cell borders disappeared.…”

“…Also, the treatment caused other damage in the midgut, with some epithelial cells showing symptoms of shrinking and others of swelling; the appearance of vacuoles in epithelial cells was accompanied by the destruction of the gut wall and epithelial cell borders disappeared. The same results were obtained in Simulium pertinax larvae exposed to delta-endotoxins of Bacillus thuringiensis (Cavados et al, 2004), in Blattella germanica exposed to boric acid (Habes et al, 2006), in Chrysomya megacephala fed on malathion (Bakr et al, 2012), in Periplaneta americana exposed to N-nitroso-Nmethyl urea (Jain and Ahi, 2014), in Periplaneta americana exposed to deltamethrin (Majumdar et al, 2016), in Culex pipiens larvae treated with Carum copticum methanol extract (Al-Mekhlafi, 2018), in Dysdercus koenigii exposed to deltamethrin (Faizan et al, 2019), and in Sarcophaga ruficornis exposed to cypermethrin (Mirza and Amir, 2022). Ethical Approval:All experiments in this research were approved by the Research Ethics Committee of the Faculty of Science, South Valley University, Qena governorate, Egypt.…”

Forensic Entomotoxicology: A Brief Look at Sample Preparation Techniques – A Review

“…Since then, several authors have developed methods for the analysis of the most varied types of analytes, being the vast majority in applications larvae or pupae of species of the Calliphoridae family given its importance for cases related to forensic entomology. From the onwards, several studies involving entomotoxicological analysis of Calliphoridae specimens tissues, developed only qualitative studies or even quantitative ones, without referring to any validation of the methodology developed [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44].…”