A study was conducted to investigate the effects of dietary oligofructose (1, 2 and 3%) on the blood profiles of beluga (Huso huso) juveniles (18.77 ± 0.76 g) compared to fish fed an un-supplemented diet. After 7 weeks of feeding on the experimental diets, haematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group (0% oligofructose), dietary oligofructose had no effect on red blood cell counts (RBC), mean corpuscular volume (MCV), mean cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) (P > 0.05). However, haemoglobin (Hb) concentration, leucocyte (WBC) levels and the proportion of lymphocytes were significantly higher (P > 0.05) in the 2% oligofructose fed fish than in the 3% oligofructose fed fish. Additionally, haematocrit (Hct) values (P = 0.049) and the proportion of lymphocytes (P ≤ 0.01) were significantly higher in the 2% oligofructose group than in the control group. Although serum glucose and total protein remained unaffected, serum cholesterol was significantly lower in the 2% oligofructose group than in the control and 3% oligofructose group (P < 0.05). The results of the present study showed that oligofructose had no significant effects on serum lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. These results indicate that fish blood profiles could be affected by prebiotics, which should be taken into account in future studies.
The stocks of Caspian roach (Rutilus rutilus), an economically important species in the Caspian Sea, is depleting. Annually, millions of fingerlings of these species are produced artificially and restock in the mouth of Qare Soo River in the Southern Caspian Sea, where they exposed to the pesticides coming from rice and orchard farms in the region. The fate of this exposure in hypo-osmoregulatory ability of juvenile fish is important. Therefore, this study initially investigates fingerlings of Caspian roach which was exposed to different concentrations of diazinon (close to estuaries concentration) for 96 h, then they were transferred to the brackish water for 240 h where the growth and survival rate were examined. The major changes in the exposed fish gills to pesticides were collapsed secondary lamellae, oedema, epithelial lifting, and shortening of secondary lamellae. The findings of this study showed decrease of tissue damage in the gill tissue in fingerlings at brackish water, while, a lower growth rate was seen compared to the control group (P < 0.05). This study suggests that the exposure of Caspian roach fingerlings to diazinon in fresh water may jeopardize their physiological capabilities and subsequent survive in brackish water conditions which may cause failure in fish stocks rebuild project in the Caspian Sea.
The effects of heparin and ethylenediaminetetraacetic acid (EDTA) on plasma biochemistry and hematological parameters in Asipencer persicus were studied. Differences in ALT and ALP activity were found between serum and heparinized plasma (P<0.05). AST activity in EDTA treated samples increased (P<0.05) compared to its values in serum. On the contrary, ALP activity showed a significant decrease compared to its serum level (P<0.05). Samples collected with EDTA showed an increase in haematocrit, mean corpuscular volume (MCV) and white blood cells (WBC) values (P<0.05) when compared to those collected in heparin. The other measured biochemical parameters did not show any variation. The present study suggests the use of heparin as a preferred anticoagulant for routine haematological analyses in the Persian sturgeon. However, both of these anticoagulants inflicted changes in selected biochemical parameters.
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