Comparative study of hematological and blood chemistry of Persian sturgeon (Asipencer persicus) exposed to two common anticoagulants

Abstract

The effects of heparin and ethylenediaminetetraacetic acid (EDTA) on plasma biochemistry and hematological parameters in Asipencer persicus were studied. Differences in ALT and ALP activity were found between serum and heparinized plasma (P<0.05). AST activity in EDTA treated samples increased (P<0.05) compared to its values in serum. On the contrary, ALP activity showed a significant decrease compared to its serum level (P<0.05). Samples collected with EDTA showed an increase in haematocrit, mean corpuscular volume (MCV) and white blood cells (WBC) values (P<0.05) when compared to those collected in heparin. The other measured biochemical parameters did not show any variation. The present study suggests the use of heparin as a preferred anticoagulant for routine haematological analyses in the Persian sturgeon. However, both of these anticoagulants inflicted changes in selected biochemical parameters.

Effect of anticoagulants on farmed giant kokopu, Galaxias argenteus (Gmelin 1789) haematological parameters and erythrocyte fragility

“…The fact that citrate appeared to cause anisonucleosis, lysis and karyolysis may suggest its unsuitability for G. argenteus blood treatment (Figure 1a). K 2 EDTA has been reported in several studies to induce erythrocyte haemolysis, karyolysis, anisonucleosis, anisocytosis, cell swelling and elevation of haematocrit values, among other effects (Ahmed & Maqbool, 2014; Farias et al ., 2016; Gholipourkanani, Ranjdoost, Jafaryan, & Yelghi, 2018; Lataretu, 2013; Maqbool, Ahmed, & Sheikh, 2013; Walencik & Witeska, 2007; Witeska & Wargocka, 2011). Except for karyolysis and lysis, most conditions were not observed in K 2 EDTA‐treated erythrocytes in this study (Clark et al ., 1979; Ishikawa et al ., 2010; Lataretu, 2013; Tavares‐Dias & Sandrim, 1998).…”

Blood-Chemistry Parameters Comparison among Different Age Stages of Chinese Sturgeon Acipenser sinensis

“…Blood parameters play a crucial role in animals' circulatory systems, serving essential functions such as material transport, physiological regulation, and defense mechanisms [12]. They act as parameters of species characteristics and physiological status, reflecting animal health and nutritional levels [13], and provide valuable insights for research in animal reproductive physiology and pathology.…”