A total of 21 (4.3%)
enterohemorrhagic E. coli strains were isolated by biochemical tests and
identification of the eae+stx1+stx2+
genotype from 490 stool samples obtained from calves with diarrhea during 1-year period
from a major farm in Tehran, Iran. All of the strains showed resistance to ampicillin,
ciprofloxacin, trimethoprim, streptomycin, chloramphenicol and tetracycline, while 19%
showed resistance to gentamicin. Out of 21 EHEC strains, 11 (53%) harbored class 1
integron. Two different amplification products, which were approximately 750 and 1,700 bp
in size, were obtained from amplified variable regions
(in-F/in-R primers) in 3 (14.3%) and 4 (19%) of the
EHEC isolates, which corresponded to dfrA7(dihydrofolate reductase type
I) and dfrA1/aadA1(dihydrofolate
reductase/aminoglycoside adenyltransferase) resistance gene cassettes, respectively, and
this was confirmed by sequencing. Genotyping analysis revealed a total of 16 pulsotypes
that corresponded to 16 isolates with the similarity indices of 62% and 30% for the most
and least similar isolates, respectively, 9 of which harbored class 1 integron. Analysis
of pulsotypes showed an extensive diversity among the isolates harboring integron, which
is indicative of a lack of any significant genetic relatedness among the isolates. No
obvious relation could be deduced between integron content and special pulsotypes. The
little data available on the genotyping patterns of EHEC isolates from cattle and their
resistance gene contents emphasize the need to establish genotyping databases in order to
monitor and source track the source of emergence and spread of new resistant and
integron-carrying genotypes.
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