Detection of VIM- and IMP-type Metallo-Beta-Lactamase Genes in Acinetobacter baumannii Isolates from Patients in Two Hospitals in Tehran

Davoodi, Saba; Boroumand, Mohammad Ali; Sepehriseresht, Saeed; Pourgholi, Leyla

doi:10.15171/ijb.1088

Cited by 11 publications

(5 citation statements)

References 16 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indeed, we detected the gene coding for the VIM in 56% of strains, a higher rate than in other studies at 5.7% [ 20 ] and 7% [ 21 ]. However, this is slightly consistent with that reported by PeshattiWar et al, the rate being noted at 62.5% [ 22 ]. Moreover, we detected the gene coding for SIM in 39% and the gene encoding for GIM in 37%.…”

Section: Discussionsupporting

confidence: 92%

Acinetobacter baumannii Carbapenemase Producers in Morocco: Genetic Diversity

Massik,

Hibaoui,

Benboubker

et al. 2023

Cureus

View full text Add to dashboard Cite

Introduction: Carbapenem resistance in Acinetobacter baumannii ( A. baumannii ) is a public health problem worldwide. Although carbapenem resistance is emerging in Morocco, few studies have shown the epidemiological profile of carbapenemase genes in Moroccan healthcare facilities. The aim of this study was to characterize the molecular profile of the carbapenemase enzyme in Acinetobacter baumannii from clinical isolates. Methods: Clinical strains isolated in the laboratory from various samples were subjected to several phenotypic tests. Antibiotic susceptibility and identification were tested using Phoenix 100 (Becton Dickinson Co., Sparks, MD, USA) and Api 20 (bioMérieux, Marcy-l'Etoile, France). Simple phenotypic assays were used to detect carbapenemase oxacillinase (OXA) and metallo-β-lactamase (MBL) production, including the modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA) test. The detection of carbapenemase genes was performed by multiplex and simple polymerase chain reaction (PCR). Results: A total of 140 strains or 100% of isolates contained OXA-51 and ISbA1 sequences, 89% contained OXA-23 and OXA-58 sequences, and 1% contained OXA-24 sequence. The MBL genes were predominated by Verona integron-encoded metallo-β-lactamase (VIM) (56%), followed by Seoul imipenemase (SIM) (39%), German imipenemase (GIM) (37%), São Paulo metallo-β-lactamase (SPM) (13%), imipenemase (IMP) (11%), and New Delhi metallo-β-lactamase (NDM) (4%). Guyana extended-spectrum β-lactamase (GES) was not found in any isolation. Conclusion: Our study shows a high frequency of carbapenem resistance in Acinetobacter baumannii , as it reports a high molecular diversity of carbapenemase-encoding genes, mainly dominated by the carbapenemase ISaba1/OXA-23, which represents an emerging threat in our hospital.

show abstract

Section: Discussionsupporting

confidence: 92%

Acinetobacter baumannii Carbapenemase Producers in Morocco: Genetic Diversity

Massik,

Hibaoui,

Benboubker

et al. 2023

Cureus

View full text Add to dashboard Cite

show abstract

“…We found that the gene coding for VIM is predominant by 55.7%, this rate is higher compared to other studies ( 24 , 25 ) which reported respectively rates of 5.7% and 7%. However, it is still slightly in agreement with that reported by PeshattiWar et al ( 26 ) indicating a percentage of 62.5%. The first MBL identified in A. baumannii strains was the IPM-2 reported in 2000 in Italy ( 27 ).…”

Section: Discussionsupporting

confidence: 90%

First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco

Massik¹,

Hibaoui²,

Benboubker³

et al. 2022

IJM

View full text Add to dashboard Cite

Background and Objectives: Carbapenem-resistant Acinetobacter baumannii has recently been identified by the World Health Organization as a critical pathogen. We propose to characterize the molecular characteristics of clinical isolates of A. baumannii resistant to carbapenems collected in a Moroccan hospital. Materials and Methods: Seventy carbapenem-resistant A. baumannii isolates from various samples were received at the mi- crobiology laboratory of the Hospital Center. Antibiotic susceptibility was tested by the diffusion disc method and molecular characterization of antimicrobial resistance was performed by PCR and sequencing. Results: Carbapenemase genes were detected in our isolates: the OXA-51 gene and the ISbA1 sequence were detected in all isolates (100%), the OXA-23 and OXA-58 genes were detected in 82.85% and 10% of isolates respectively, MBL genes were dominated by VIM 39 isolates (55.7%), followed by GIM 26 isolates (37%), SIM 20 isolates (28.5%), IMP 8 isolates (11, 4%), NDM 3 isolates (4%) and for the first time in Morocco SPM with 4 isolates (5.7%). Conclusion: The emergence of resistance of A. baumannii to carbapenems is a serious problem in our hospital which re- quires the establishment of a prevention strategy and strict respect for hygiene to minimize their dissemination.

show abstract

“…The nontransferable characteristic of bla VIM-1 in these isolates suggests chromosomal or a nonconjugative plasmid. The gene was also detected in eight out of 13 strains tested in a study in Saudi Arabia and Iran where bla VIM-2 was also present [285][286][287]. Both bla VIM-1 and bla VIM-2 were also detected in a study of 100 A. baumannii isolates from three teaching hospitals in Iran [288].…”

Section: Vim Groupmentioning

confidence: 99%

Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace

2020

View full text Add to dashboard Cite

Acinetobacter baumannii is a common cause of serious nosocomial infections. Although community-acquired infections are observed, the vast majority occur in people with preexisting comorbidities. A. baumannii emerged as a problematic pathogen in the 1980s when an increase in virulence, difficulty in treatment due to drug resistance, and opportunities for infection turned it into one of the most important threats to human health. Some of the clinical manifestations of A. baumannii nosocomial infection are pneumonia; bloodstream infections; lower respiratory tract, urinary tract, and wound infections; burn infections; skin and soft tissue infections (including necrotizing fasciitis); meningitis; osteomyelitis; and endocarditis. A. baumannii has an extraordinary genetic plasticity that results in a high capacity to acquire antimicrobial resistance traits. In particular, acquisition of resistance to carbapenems, which are among the antimicrobials of last resort for treatment of multidrug infections, is increasing among A. baumannii strains compounding the problem of nosocomial infections caused by this pathogen. It is not uncommon to find multidrug-resistant (MDR, resistance to at least three classes of antimicrobials), extensively drug-resistant (XDR, MDR plus resistance to carbapenems), and pan-drug-resistant (PDR, XDR plus resistance to polymyxins) nosocomial isolates that are hard to treat with the currently available drugs. In this article we review the acquired resistance to carbapenems by A. baumannii. We describe the enzymes within the OXA, NDM, VIM, IMP, and KPC groups of carbapenemases and the coding genes found in A. baumannii clinical isolates.

show abstract

Detection of VIM- and IMP-type Metallo-Beta-Lactamase Genes in Acinetobacter baumannii Isolates from Patients in Two Hospitals in Tehran

Cited by 11 publications

References 16 publications

Acinetobacter baumannii Carbapenemase Producers in Morocco: Genetic Diversity

Acinetobacter baumannii Carbapenemase Producers in Morocco: Genetic Diversity

First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco

Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace

Contact Info

Product

Resources

About