Background In the past, there has been an exponential increase in the potential biomarkers that can be used for staging of liver fibrosis. In light of intraobserver and intralobular variations, criticism has been directed at liver biopsy, and its efficacy has been challenged. Shear-wave elastography (SWE) has become a routine method for pre-assessment of liver fibrosis. Serum markers such as chitinase-3-like protein 1 (CHI3L1) also known as YKL-40, aspartate aminotransferase-to-platelet ratio index, and fibrosis-4 (Fib-4) index have been researched as potential alternates to detect liver fibrosis. Study A total of 150 enrolled patients with chronic hepatitis underwent serum analysis to estimate CHI3L1 or YKL-40 level, aspartate aminotransferase-to-platelet ratio index, and Fib-4 index. These patients also underwent SWE. Results The distribution of fibrosis grade according to SWE was F0: 46 patients, F1: 31 patients, F2: 16 patients, F3: four patients, and F4: 53 patients. Receiver operating characteristic curve analysis for F0–F1 versus F2–F3, F0–F1 versus F4, and F2–F3 versus F4 gave area under curve values of 0.56 (P>0.05), 0.76 (P<0.01), and 0.75, respectively (P<0.01) for aspartate aminotransferase-to-platelet ratio index; of 0.65 (P<0.05), 0.78 (P<0.01), and 0.7, respectively (P<0.05) for Fib-4 index; and 0.98, 0.99, and 0.95, respectively (P<0.01 for all) for CHI3L1. Conclusion CHI3L1 could be used as a preliminary tool to assess mild/absent fibrosis from significant fibrosis and cirrhosis.
Analyse des sérotypes du virus de la dengue par amplification en chaîne par polymérase multiplexe pendant la flambée de dengue de 2015 à Rawalpindi (Pakistan) RÉSUMÉ La dengue est l'une des plus importantes maladies virales transmises par des arthropodes. Elle est endémique dans plus de 125 pays dont le Pakistan, l'incidence mondiale étant comprise entre 50 et 200 millions. Nous avons déterminé la fréquence des différents sérotypes du virus de la dengue pour mettre en évidence son hyperendémicité à Rawalpindi (Pakistan). Les échantillons de sérum de 140 patients susceptibles d'avoir contracté le virus de la dengue ont été analysés entre mai et octobre 2015 à l'aide du test ELISA et de l'amplification en chaîne par polymérase multiplexe. Cent huit patients étaient infectés par le sérotype 2, 16 par le sérotype 3, sept par le sérotype 4 et trois par le sérotype 1. Trois patients étaient infectés par les sérotypes 1 et 2, un patient par les sérotypes 1 et 4 et un autre par les séroptypes 2 et 3. L'incidence de la dengue a considérablement augmenté ces 50 dernières années et elle s'est étendue à des zones qui étaient précédemment exemptes de la maladie. Le sérotype 2 était prédominant dans notre population suivi par le sérotype 3. Il n'existe actuellement aucun traitement spécifique pour la dengue. La lutte antivectorielle et la vaccination constituent les seules méthodes efficaces pour prévenir les flambées futures. ABSTRACT Dengue is one of the most important arthropod-borne viral diseases. It is endemic in > 125 countries including Pakistan, with a global incidence of 50-200 million. We determined the frequency of different serotypes of dengue virus to highlight its hyperendemicity in Rawalpindi, Pakistan. Between May and October 2015 we analysed the serum samples of 140 patients with a suspicion of dengue, using ELISA and multiplex polymerase chain reaction. One hundred and eight were infected with serotype 2, 16 with serotype 3, 7 with serotype 4 and 3 with serotype 1. Three patients were infected with serotypes 1 and 2, and 1 each with serotypes 1 and 4 and serotypes 2 and 3. Incidence of dengue has increased many fold in the past 50 years and has expanded to areas that were previously free from the disease. Serotype 2 was predominant in our population followed by serotype 3. There is currently no specific treatment for dengue, and vector control and vaccination are the only effective methods to prevent future outbreaks.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.