An X-ray diffractometer with a mobile emitter-detector system is able to perform various X-ray examinations of a stationary sample. The design for this instrument is described, along with its operating principles and control programs. Owing to its high-precision angular movement sensors and its two detectors (one of these, a linear position-sensitive detector, has a working length of 100 mm and a resolution of <0.2 mm), the diffractometer can be used in both traditional and nontraditional experiments on X-ray reflection, scattering, and absorption. Results from using this instrument in reflectometry and X-ray tomography are presented as an example. Fig. 1. External appearance of the diffractometer with a mobile emitter-detector system.
Histological and morphometrical analysis of the brain and peripheral analyzers revealed proliferative migration abnormalities of brain development and neuron differentiation in mammals during a space flight, which can limit space exploration.
The appearance of autoantibodies to neuronal proteins (S100, GFAP, MBP, and NGF) in rat serum was analyzed by ELISA on days 5, 10, 17, 25, and 32 after streptozotocin injection. Simultaneously, blood glucose and insulin autoantibodies were assayed. Serum glucose level increased on the next day after streptozotocin injection and the level of autoantibodies to insulin significantly increased on day 5 indicating the development of diabetes. The levels of antibodies to specific neuronal proteins (S100, GFAP, MBP, and NGF) also increased at this term. It is concluded that diabetes with streptozotocin is associated with damage to the blood-brain barrier.
Schwann cells forming peri-insular glial sheath of the pancreatic islets in some mammals can be involved in the pathogenesis of diabetes mellitus. Human pancreatic cells contain small elongated or oval cells of unknown origin with S100-immunopositive processes. We found that cells with processes located in pancreatic islets of human fetuses and children are similar to Schwann cells by their morphological and ultrastructural characteristics, immunopositive reaction for S100, and integration with nerve endings. In the pancreas of fetuses and children, Schwann cells are often seen in forming pancreatic islets and around the pancreatic ducts. The data suggests that Schwann cells can participate in the morphogenesis of human pancreatic islets.
The study was carried out within the framework of a Russian-Canadian experiment aboard the Bion-10 satellite. The volume and surface area of the gray and white matter and ventricles of the brain, retina, olfactory placodes, the VIII nerve ganglia, and vascular plexus were measured in Xenopus laevis which had been in a state of weightlessness for 2 weeks since their hatching. Zero gravity was found to stimulate the growth of nerve processes, to increase the surface of the vascular plexus, and to impede the development of the retina, olfactory placodes, and VIII nerve ganglia.
Integral cytophotometry was used to measure lactate dehydrogenase (LDH) and its H-and M-isoforms in neurons and satellite gliocytes in tissue sections from the cranial cervical sympathetic ganglion of the rabbit in normal conditions and after experimental partial and complete pharmacological blockade of nicotinic cholinoreceptors (n-CR). In normal conditions, both cell types showed both the H-and M-type isoforms, though the isoenzyme profiles differed--neurons showed a dominance of H-isoform activity while the M-isoform was more active in satellite gliocytes. In partial and complete blockade, the activity of LDH and its H-and M-isoforms decreased significantly in proportion to the number of blocked n-CR. In satellite gliocytes, increases in the extent of blockade were associated with decreases in the activity only of the M-isoform, while the activity of the H-isoform did not change. In partial blockade, the LDH isoenzyme profile of satellite gliocytes shifted towards the neuronal isoform, while in complete blockade there was no difference from the LDH isoenzyme profile of intact neurons. These data led to the suggestion that the formation of lactate in satellite gliocytes is induced by nicotinic cholinergic synapses directly involved in neuron-glial interactions and in controlling the activity of the LDH enzyme system in sympathetic neurons.
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