The aim: Of the work was to determine the content of pro- and anti-inflammatory cytokines in the blood serum of the control group rats and after 28 days of inhibiting HCl secretion in the stomach by proton pump blockers “Omeprazole” and “Pantoprazole”. Materials and methods: The studies were performed on 30 white non-linear male rats weighing 160-180 g, divided into three groups with 10 animals in each. The control (group 1) were injected intraperitoneally with water for injections within 28 days once a day. Group 2 was administered omeprazole. Group 3 was administered pantoprazole. The concentration of cytokines in the blood serum of rats was determined by the enzyme immunoassay method. For statistic data processing, Student’s t-criterion for independent samples was applied. Results: After prolonged administration of omeprazole and pantoprazole, the blood serum concentrations of IFNγ, TNFα, IL-1 in rats increased by 58.5% and 3.41%, 73.3% and 48.4%, 80.2% and 40.8%, respectively, and IL-12B 40p decreased by 36.6% when using omeprazole and was almost indistinguishable from the control values when pantoprazole was administered. With administration of omeprazole, IL-4 concentration decreased by 39.8% and that of pantoprazole increased by 3.86% compared to the control. Administration of omeprazole and pantoprazole did not affect IL-6 concentration. Conclusion: Inhibition of hydrochloric acid secretion in the stomach of rats for 28 days using omeprazole and pantoprazole led to an imbalance between pro- and anti-inflammatory cytokines. The adverse effect of pantoprazole was less pronounced than that of omeprazole.

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The activity of antioxidant protection enzymes in the blood serum and colon mucosa in rats was studied under the conditions of 28-days administration of omeprazole on its own and omeprazole together with multiprobiotics "Symbiter" and "Apibact". Physiological and biochemical study methods were applied. It was found that after omeprazole administration, the activity of superoxide dismutase in the blood serum decreased, and the activity of catalase increased compared to the control. With the co-administration of omeprazole and multiprobiotics, the activity of superoxide dismutase increased compared to the group of rats that received omeprazole only during the same time, but remained less compared to the control group. The content of reduced glutathione in the blood serum of rats after administration of omeprazole decreased, the activity of glutathione peroxidase and glutathione transferase increased, and the activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics, the serum RG content was at the control level, the activity of glutathione reductase exceeded the control values. The activity of glutathione reductase decreased compared to the group receiving omeprazole only. The activity of glutathione reductase increased and did not differ from the control values. In the colon mucosa, superoxide dismutase and catalase activity decreased compared to control. With the combined administration of omeprazole and multiprobiotics, superoxide dismutase and catalase activity increased and even exceeded the control values. With the administration of omeprazole, the reduced glutathione content in the colon mucosa was lower than that in the control. The activity of glutathione peroxidase increased and glutathione transferase and activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics to rats, the reduced glutathione content increased compared to the group of rats administered omeprazole only, and even exceeded that in the control.

Полтавський національний педагогічний університет ім. В. Г. Короленка, Україна pilipenko_s@ukr.netВстановлено, що після 28 днів введення омеп-разолу концентрація гастрину в сироватці крові зростала в 3,1 раза (p<0,05). Кількісний і якісний склад мікрофлори шлунка після 28 днів введення омепразолу зазнавав суттєвих змін у 40% піддос-лідних щурів лактобактерії зовсім не висівались. При цьому більше, ніж у 100 разів (p<0,05) зроста-ла колонізація шлунку умовно-патогенною мікроф-лорою та грибами роду Candida. На фоні тривалої гіпоацидності шлункового соку шлунок заселився умовно-патогенними бактеріями, а саме Klebsiella, Staphylococcus aureus, Staphylococcus epidermidis (з гемолізом), концентрація яких була значною і складала 10 4 -10 5 КУО/г. В 10 разів (p<0,05) зросла концентрація Enterobacter.Сумісне введення щурам упродовж 28-ми днів омепразолу і мультипробіотиків «Симбітер ацидо-фільний» концентрований і «Апібакт» усувало роз-виток дисбактеріозу в шлунку і товстій кишці, що проявлялось у відновленні кількісного і якісного складу мікрофлори, а також зменшувало концент-рацію гастрину в сироватці крові на 24,9% (p<0,05) та 43,0% (p<0,05), відповідно, у порівнянні з групою щурів, які отримували один омепразол.Ключові слова: гіпохлоргідрія, гіпергастрине-мія, дисбактеріоз, пробіотики.

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In the article the results of dynamic running and traction-energy tests of the electric locomotive VL40U are presented. In accordance with the test results a conclusion about the suitability of electric locomotive of such a type for operation with trains containing up to 15 passenger coaches inclusive is made.

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