Influence of the Durable Application of Protonic Pump Inhibitors on Cytokine Concentration in Rat Blood Serum

Abstract

The aim: Of the work was to determine the content of pro- and anti-inflammatory cytokines in the blood serum of the control group rats and after 28 days of inhibiting HCl secretion in the stomach by proton pump blockers “Omeprazole” and “Pantoprazole”. Materials and methods: The studies were performed on 30 white non-linear male rats weighing 160-180 g, divided into three groups with 10 animals in each. The control (group 1) were injected intraperitoneally with water for injections within 28 days once a day. Group 2 was administered omeprazole. Group 3 was administered pantoprazole. The concentration of cytokines in the blood serum of rats was determined by the enzyme immunoassay method. For statistic data processing, Student’s t-criterion for independent samples was applied. Results: After prolonged administration of omeprazole and pantoprazole, the blood serum concentrations of IFNγ, TNFα, IL-1 in rats increased by 58.5% and 3.41%, 73.3% and 48.4%, 80.2% and 40.8%, respectively, and IL-12B 40p decreased by 36.6% when using omeprazole and was almost indistinguishable from the control values when pantoprazole was administered. With administration of omeprazole, IL-4 concentration decreased by 39.8% and that of pantoprazole increased by 3.86% compared to the control. Administration of omeprazole and pantoprazole did not affect IL-6 concentration. Conclusion: Inhibition of hydrochloric acid secretion in the stomach of rats for 28 days using omeprazole and pantoprazole led to an imbalance between pro- and anti-inflammatory cytokines. The adverse effect of pantoprazole was less pronounced than that of omeprazole.

Laryngeal and Esophageal Mucosal Protection Using the Angico Gum Biopolymer in a Mouse Model of Reflux

“…Thus, we observed a similar effect with angico gum. Results obtained for omeprazole, which is a potent proton pump inhibitor, were similar to those for angico gum in terms of wet weight and MPO activity due to the anti‐inflammatory effect of PPIs 29 …”

“…Results obtained for omeprazole, which is a potent proton pump inhibitor, were similar to those for angico gum in terms of wet weight and MPO activity due to the anti-inflammatory effect of PPIs. 29 Regarding mucosal integrity, angico gum prevented the loss of transepithelial electrical resistance and paracellular permeability, like omeprazole did. In this context, studies have demonstrated that cashew gum is also able to prevent the loss of mucosa in the larynx.…”