The transevaporator sampling technique is described for the solvent stripping of the organic volatile fraction from 5-500-pl samples of biological fluids prior to analysis by capillary column gas chromatography. The reproducible nature of the isolation and separation technique enables quantitative comparisons to be made between nQrmal and pathological samples and marker substances of use for the diagnosis of the diseased state to be identified. Electron-capture detector profiles provide complementary information to the flame-ionisation detector that may prove to be very useful in developing biological correlations.
The response of the electron-capture detector to organic compounds is poorly defined, and the steroids are no exception to this observation. For those steroids which are naturally electron-capturing, the structures of the electrophores will be defined. Other steroids can be made electron-capturing by the formation of appropriate derivatives. Some new or infrequently used reagents for this purpose (flophemesyl ethers, t-bulflophemesyl ethers, pentafluorophenylhydrazone derivatives and halogen-substituted aromatic boronic acids) are described.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.