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The purpose of this in vitro study was to determine the antibacterial effects of leaf extract of koi (Streblus asper) against six anaerobic bacteria: Porphyromonas gingivalis W50, Prevotella intermedia, Actinomyces naeslundii (T14V), Peptostreptococcus micros, Actinobacillus actinomycetemcomitans ATCC 43717 and ATCC 43718. The dried-pulverized leaves were extracted using 50% (v/v) redistilled ethanol. Employing the disc diffusion method, it was demonstrated that 15 µL of the leaf extract at 250 and 500 mg/mL had inhibitory effects towards all bacterial strains tested except A. actinomycetemcomitans ATCC 43717. The minimum inhibitory concentration of S. asper leaf extract on P. gingivalis W50, A.