Background: Sweet sorghum (Sorghum saccharatum (L.) Moench) is a unique crop with great potential to serve both the food and energy industries. It is due to the possibility of (bio)ethanol production both from the juice and biomass of this crop. The sorghum stems juice contains sugar in the levels similar to that of sugarcane. Besides, low cultivation requirements for the sweet sorghum make this crop even more attractive for sugar and ethanol production. In terms of technology, sweet sorghum is seen as a transitional feedstock for the first to the second generation bioethanol production. However, effective technological development of the plant cultivation and processing in the Northern and Central Ukraine is restrained by the lack of a collection of sweet sorghum genotypes and adapted varieties for its large-scale cultivation. Additionally, no evaluations of potential (bio)ethanol productivity have been performed for this region, which is important for efficient implementation of novel biofuel-producing technologies and for successful development of a green economy. Objective: This research was aimed to create a pool of sweet sorghum genotypes with the involvement of worldwide germplasm, analyze their morphology and breed high-yielding plant lines for the efficient production of liquid biofuels for second-generation bioenergy. Based on that, we also aimed to explore the prospects regarding the efficiency of sweet sorghum cultivation for (bio)ethanol production in the Northern and Central Ukraine. Methods and Materials: A valuable gene pool of S. saccharatum (L.) Moench (41 samples) was created; in particular, high-performance genotypes were created for cultivation under the soil-climatic conditions of Ukraine. The bio-morphological features and the yield potential of the plants were determined and the biochemical composition of the phyto-raw materials was determined in different periods of vegetation, in particular, during the technical ripeness of the above-ground mass of plants. The more productive forms and varieties of sugar sorghum in terms of yield, dry matter content, sugar, and energy value of biomass during flowering and waxy ripeness are highlighted. The technological properties of plant biomass for the production of alternative liquid fuels (in particular, bioethanol) have been analyzed. Importantly, optimal cultivation conditions have been elaborated for the newly created sweet sorghum genotypes, and their productivity has also been evaluated. Moreover, for the first time, a detailed study on potential ethanol yield has been conducted. Results: Sweet sorghum has considerable potential in Ukraine as a new sugar-producing energy crop. The germplasm collection of this crop has been created (41 accessions), including introduced and acclimated genotypes and newly bred lines and varieties. The biological performance of sorghum in Ukraine and plant morphology have been analyzed. The most promising genotypes were used for breeding of new high-productive sweet sorghum varieties. The potential (bio)ethanol yield for different sugar feedstocks (juice, grain bagasse) can reach up to 11423 L/ha in total from juice, grain and bagasse. Conclusion: The estimated values of ethanol productivity are comparable to the results of other similar investigations. In conclusion, a high performance of sweet sorghum in Ukraine can be suggested.
Results of obtaining miscanthus polyploid lines using both classic and new dinitroanilines to improve productivity of biomass as well as quality for biofuel production are represented. All of compounds applied in the study appeared to be able to induce poyploidy in Miscanthus × giganteus.It was found that new antimitotics have a lower phytotoxicity level compared to classic dinitroani-lines. Ploidy level of obtained lines was confirmed by cytological studies using light microscopy. These lines are acclimatised for growing in open soil conditions. Currently, morphometric parameters of selected lines are being studied and analysed.
Aim. Main aim of this research was a comparison of fatty acid composition for seed oils from of turnip rape breeding forms and varieties produced in M.M. Gryshko Natl. Botanical Garden of Natl. Academy of Sciences of Ukraine. Methods. Biochemical analysis of oil content as well as chromatographic analysis of fatty acid composition of mentioned above turnip rape genotypes were conducted out. Results. Oil content in seeds of spring (Brassica campestris f. annua D.C.) and winter (B. campestris f. biennis D.C.) turnip rape forms and varieties was determined. The highest oil content was indicated for winter variety Oriana – 38.1 %. Basing on chromatographic analysis indicated two types of fatty acid composition: high-erucic with content of 22:1 fatty acid up to 42.8 % and high-oleic with content of 18:1 fatty acid up to 46.92 %. Conclusions. Taking in account results of chromatographic analysis and agronomic productivity the best genotypes for biodiesel production were identified: winter variety Oriana and spring form EOSYaF-1. Chosen genotypes could be used for production of two different types of this biofuel – “light” and “heavy”, – according to difference in fatty acid composition.Keywords: Brassicaceae, turnip rape, spring varieties, winter vatieties, oil, fatty acids, composition, biodiesel.
Aim. To conduct a comprehensive study of morphological features of the genus Vigna plants, to assess their productive potential depending on the species characteristics under the conditions of introduction in the Right-Bank Forest-Steppe of Ukraine. Methods. The main method of work was a comparative morphological analysis of plants grown from seeds. The research was carried out at the introduction sites and in the laboratory of the Department of Cultural Flora of the M. M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine during 2019–2021. Plants of four species of the genus Vigna: V. radiata (L.) R.Wilczek, V. angularis (Willd.) Ohwi & H.Ohashi, V. mungo (L.) Hepper, V. unguiculata (L.) Walp were the material for the study. We studied the patterns of growth processes and plant development depending on species characteristics. Soil germination of seeds without prior preparation was determined depen ding on species characteristics. Field, laboratory and me thods of analysis of variance and statistical evaluation of average data using Microsoft Excel (2010) were used. Results. It was revealed that the seeds of plants V. radiata, V. angularis, V. mungo, V. unguiculata being introduced in the Right Bank Forest-Steppe of Ukraine were characterized by a high level of sprouting energy (from 64.8% in V. angularis to 78.9% in V. unguiculata ) and germination (from 84.3% in V. radiata to 99.6% in V. angularis), the intensity of growth and development of aboveground and underground organs during the growing season and seed productivity (from 468 g/m2 in V. radiata to 585 g/m2 in V. mungo). This indicates the prospects of their cultivation as a source material for breeding research and the advisability of use in crop production, which will expand the potential of the raw material base in addition to traditional legumes and cereals. Conclusions. The morphological characteristics of seeds, vegetative and reproductive organs of plants of the genus Vigna were studied. Peculiarities of formation of seed productivity, aboveground and underground part of plants during vegetation depending on species specificity in the conditions of introduction in the Right-Bank Forest-Steppe of Ukraine were determined.
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