Induction of Polyploidy in Giant Miscanthus (<i>Miscanthus × Giganteus</i> Greef Et Deu.)

Melnychuk, Oleksandr; Ozheredov, S. P.; Rakhmetov, Dzhamal; Shysha, Olena O.; Rakhmetova, S. O.; Yemets, А. І.; Blume, Yaroslav B.

doi:10.2478/prolas-2020-0032

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…Regeneration of hexaploids of Miscanthus x giganteus from immature inflorescences derived callus treated with the antimitotic agents colchicine and oryzalin in liquid and solid media was reported by Yu et al [25]. Melnychuk et al [26] used for polyploidization induction dinitroanilines as antimitotic agents.…”

Section: Introductionmentioning

confidence: 93%

In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators

Blinstrubienė

Jančauskienė

Burbulis

2021

Plants

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Miscanthus x giganteus is a spontaneous sterile hybrid therefore the creation of useful genetic diversity by conventional breeding methods is restricted. Plant regeneration through indirect organogenesis may be a useful approach to create genetic variability of this important agricultural crop. The present study aimed to evaluate the effect of the explant type and growth regulators on indirect organogenesis of Miscanthus x giganteus and to determine the ploidy level of plant regenerants by flow cytometry. On average, the highest percentage of morphogenic callus tested explants formed in the medium supplemented with 2.5 mg L–1 IBA + 0.1 mg L–1 BAP + 4.0 mg L–1 l-proline. The most intensive secondary differentiation of callus cells was observed in the medium supplemented with 4.0 mg L–1 ZEA + 1.0 mg L–1 NAA. The highest root formation frequency with the highest number of roots was determined in the MS nutrient medium supplemented with 0.4 mg L–1 IBA, where more than 95% of plant regenerants survived and were growing normally.

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Section: Introductionmentioning

confidence: 93%

In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators

Blinstrubienė

Jančauskienė

Burbulis

2021

Plants

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“…For this species, classical breeding methods to improve economic characteristics cannot be applied. Hexaploid M. ×giganteus plants were reported in some studies (Głowacka et al, 2009(Głowacka et al, , 2010Melnychuk et al, 2020); however, owing to a lack of reports on the use of genotypes with double the number of chromosomes in breeding and industry, further research on the polyploidization of this species has been encouraged. Kopeć (2017) (Płażek et al, 2015).…”

Section: Regenerants As a Source Of New Plant Formsmentioning

confidence: 99%

In Vitro Culture as a Tool for Studying Plant Developmental Processes at the Physiological Level in Poland

Płażek

Dubert

2022

Acta Soc Bot Pol

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Over the last 40 years, in vitro tissue culture has developed dynamically and has become a popular technique for scientific research in the field of biology. Initially, studies were carried out to develop procedures to obtain callus cultures, cell suspensions, and protoplasts of various plant species. Over time, these cultures have been used to analyze the course of processes and mechanisms that occur at the cellular level, including the course of embryological development, formation of cellular structures, polyploidization, signal transduction, gene expression, and responses to various stress factors. In a minireview, different nutritional, hormonal, atmospheric, and light conditions occurring in in vitro cultures, which are stressful conditions compared to those in ex vitro plant culture, were discussed. In this review, some examples of physiological studies conducted on in vitro culture by Polish scientists are presented, including studies carried out to optimize the composition of media that induce callus and plant regeneration; determine the use of in vitro culture for the preservation of endangered plant species; understand the mechanisms of resistance responses to pathogens, salinity, nutritional stress, and low temperatures; and determine the potential production of plants with different chemical compositions. The potential of sterile plant culture is large and beyond the scope of its current use. Therefore, the number and variety of applications of these cultures will be significantly greater in the future.

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“…Раніше вже було продемонстровано можливість отримання ресинтезованих та поліплоїдних ліній міскантусу гігантського [6]. В рамках попередніх досліджень нами також було розроблено ефективні підходи для отримання поліплоїдів міскантусу гігантського і, як наслідок використання нових сполук із високою антимітотичною активністю та низькою фітотоксичністю, було успішно отримано гексаплоїдні лінії цієї культури [7]. За попередніми результатами їх польових випробувань було встановлено, що показники врожайності біомаси цих поліплоїдних ліній істотно перевищують показники триплоїдної форми [7].…”

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“…В рамках попередніх досліджень нами також було розроблено ефективні підходи для отримання поліплоїдів міскантусу гігантського і, як наслідок використання нових сполук із високою антимітотичною активністю та низькою фітотоксичністю, було успішно отримано гексаплоїдні лінії цієї культури [7]. За попередніми результатами їх польових випробувань було встановлено, що показники врожайності біомаси цих поліплоїдних ліній істотно перевищують показники триплоїдної форми [7]. Саме тому метою дослідження було проведення оцінки теоретичного виходу біоетанолу з одиниці площі гексаплоїдного міскантусу гігантського у порівнянні зі звичайною триплоїдною формою та з іншими біоетанольними культурами.…”

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Evaluation of polyploid miscanthus lines usage efficiency as a feedstock for bioethanol production

Blume¹,

Melnychuk²,

Ozheredov³

et al. 2021

Fakt. eksp. evol. org.

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Aim. Main aim of this research was the evaluation of theoretical bioethanol yield (per ha) from hexaploid giant miscanthus (Miscanthus х giganteus) and further comparison with conventional triploid form as well as with other bioethanol crops. Methods. Several mathematic functions were determined that describe yearly yield dynamics and equations, which were used in calculations of theoretical bioethanol yield. Results. The theoretical bioethanol yield was evaluated for different hexaploid miscanthus lines. The most productive in terms of ethanol yield were lines 108 and 202, from which potential bioethanol yield was found to be higher than in control line (6451 L/ha) by 10.7 % and 14.2% respectively and can reach 7144 L/ha and 7684 L/ha. Conclusions. It was determined that the most productive lines of polyploid miscanthus (lines 108 and 202) are able to compete with other plant cellulosic feedstocks for second-generation bioethanol production in Ukraine. However, these lines show bioethanol productivity than sweet sorghum, in the case when sweet sorghum is processed for obtainment of both first- and second-generation bioethanol. Keywords: bioenergy crops, biofuels, giant miscanthus, Miscanthus, polyploidy, second-generation bioethanol.

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Induction of Polyploidy in Giant Miscanthus (Miscanthus × Giganteus Greef Et Deu.)

Cited by 5 publications

References 36 publications

In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators

In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators

In Vitro Culture as a Tool for Studying Plant Developmental Processes at the Physiological Level in Poland

Evaluation of polyploid miscanthus lines usage efficiency as a feedstock for bioethanol production

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