In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators

Abstract

Miscanthus x giganteus is a spontaneous sterile hybrid therefore the creation of useful genetic diversity by conventional breeding methods is restricted. Plant regeneration through indirect organogenesis may be a useful approach to create genetic variability of this important agricultural crop. The present study aimed to evaluate the effect of the explant type and growth regulators on indirect organogenesis of Miscanthus x giganteus and to determine the ploidy level of plant regenerants by flow cytometry. On average, the highest percentage of morphogenic callus tested explants formed in the medium supplemented with 2.5 mg L–1 IBA + 0.1 mg L–1 BAP + 4.0 mg L–1 l-proline. The most intensive secondary differentiation of callus cells was observed in the medium supplemented with 4.0 mg L–1 ZEA + 1.0 mg L–1 NAA. The highest root formation frequency with the highest number of roots was determined in the MS nutrient medium supplemented with 0.4 mg L–1 IBA, where more than 95% of plant regenerants survived and were growing normally.

Bioengineering and Molecular Biology of Miscanthus

“…Huyen et al proved that MlARF-GEP is tightly related to ZmARF-GEF. Furthermore, depending on the expression patterns, in two types of callus M1ARF-GEP, this means that M1ARF-GEP in M. lutarioriparius and ZmARF-GEF in Z. mays may play similar roles [62,72].…”

Establishing in vitro cultures of Pennisetum ‘Vertigo®’ and its shoot multiplication under different LED light quality

“…In the present work, we focused on the initiation stage, including choosing the type of explant, establishing an aseptic culture, and regenerating shoots from explants cultivated in different light conditions. Recently, various biotechnological approaches have been developed for Poaceae members, in which their shoots [Blinstrubienė et al 2021, Lal 2021, immature inflorescences [Blinstrubienė et al 2021, Kopeć andPłażek 2023], seeds [Nuzhyna et al 2021, Khan et al 2022, roots [Tiécoura 2003] or leaf sheath explants [Gaikwad and Dobariya 2006] were used for in vitro induction of grass cultures. It indicates that the possibilities of obtaining plant propagule are vast in this family, but the details should be refined for specific genotypes.…”