Zophobas morio is a tropical darkling beetle which is widely exploited for commercial large-scale insect growing. Outbreaks of a disease may occur causing total devastation of cultures. In the present paper, samples of diseased Z. morio were obtained and used for establishment of a laboratory model as they were found infective to the larvae of the same insect species from another source. It took about 1 month to develop symptoms of acute disease in mid-age larvae and about twice as much when younger larvae were used for infection. Affected larvae perished quickly, and within several days up to 90-100% of the colony could perish. Both in healthy and diseased larvae a virus was detected using PCR with degenerate primers specific for a gene coding for a nonstructural protein (ORF3). The sequenced gene fragment (Genbank accession #MN732869) confirmed allocation of the virus to Densoviridae, with maximal similarity of 97.2% to Blatella germanica densovirus-like virus (#JQ320376) and 66.2% to B. germanica densovirus (#AY189948). Genomic DNA samples of Z. morio larvae from an independent colony devoid of symptoms of a disease were also positive for this virus with a slightly different (99.7% sequence similarity to the former sequence of the Z. morio densovirus) genotype (#MN732870).
Chorthippus loratus collected in Krasnodar Territory in 2017 was infected at 15% rate with a microsporidium possessing ovocylindrical binucleate spores, 2.6 9 1.2 lm in size. SSU RNA gene typing (Genbank accession # MH396491) showed its allocation to the genus Liebermannia. Degenerate primers based upon largest subunit RNA polymerase II (RPB1) sequences of closest relatives allowed amplifying the respective gene fragment of Liebermannia sp. (# MH396492). The present finding indicates worldwide distribution of the Liebermannia genus and parasitism in hosts with nonoverlapping geographic ranges (representing Neotropical versus Palearctic fauna), while previous observations were restricted to Acridoidea endemic for South America.
Beet webworm Loxostege sticticalis is a notorious pest widely distributed on the territory of Eurasia. Its outbreaks cause severe damage to crops in Russia and China. Here Wolbachia infection is reported for the first time in L. sticticalis. Larvae were sampled in Rostov, Saratov, Irkutsk Regions and Republic of Buryatia in 2005-2013. Primers targeting the wsp gene were used for the PCR screening of Wolbachia. Among 148 larvae, 35 were Wolbachia positive. Wolbachia prevalence rate ranged from 21 to 40 % in the Asian and from 0 to 47 % in the European part of Russia. The combined sample subsets were compared for European versus Asian part of Russia and 2005-2009 versus 2010-2013 timeframes. The prevalence rates of Wolbachia were not significantly different between two parts of Russia, but the endosymbiont presence (estimated for the total dataset) increased with time within the observation period.
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