Molecular genetic analysis of resistance-associated mutations in the experimental lines of spider mite<i>Tetranychus urticae</i>Koch, selected for resistance to bifenthrin and abamectin

Митина, Г. В.; Tulaeva, Irina A.; Malysh, S. M.; Tokarev, Yuri S.

doi:10.1080/01647954.2021.1990406

Cited by 2 publications

(1 citation statement)

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“…To examine whether the difference in susceptibility to bifenthrin could be explained by target-site resistance mutations, we screened for known mutations in the VGSC and found that three out of four bifenthrin-resistant strains carried the L1024V mutation in the VGSC (MR-VL, MR-VLi and ES1). Next to the L1024V mutation, the most resistant strain in this study, ES1, also showed the presence of the F1538I mutation, which has been frequently associated with pyrethroid resistance [ 16 , 17 , 18 , 19 , 30 , 91 , 92 , 93 , 94 ]. We did find the F1538I mutation in the VGSC of the susceptible strain UK4, but it was at a low frequency and therefore probably did not result in resistance in our bioassays.…”

Section: Discussionmentioning

confidence: 96%

High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

Beer

Vandenhole

Njiru

et al. 2022

Biology

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Pyrethroids are widely applied insecticides in agriculture, but their frequent use has provoked many cases of resistance, in which mutations in the voltage-gated sodium channel (VGSC), the pyrethroid target-site, were shown to play a major role. However, for the spider mite Tetranychus urticae, it has also been shown that increased detoxification contributes to resistance against the pyrethroid bifenthrin. Here, we performed QTL-mapping to identify the genomic loci underlying bifenthrin resistance in T. urticae. Two loci on chromosome 1 were identified, with the VGSC gene being located near the second QTL and harboring the well-known L1024V mutation. In addition, the presence of an L925M mutation in the VGSC of a highly bifenthrin-resistant strain and its loss in its derived, susceptible, inbred line indicated the importance of target-site mutations in bifenthrin resistance. Further, RNAseq experiments revealed that genes encoding detoxification enzymes, including carboxyl/choline esterases (CCEs), cytochrome P450 monooxygenases and UDP-glycosyl transferases (UGTs), were overexpressed in resistant strains. Toxicity bioassays with bifenthrin (ester pyrethroid) and etofenprox (non-ester pyrethroid) also indicated a possible role for CCEs in bifenthrin resistance. A selection of CCEs and UGTs were therefore functionally expressed, and CCEinc18 was shown to metabolize bifenthrin, while teturUGT10 could glycosylate bifenthrin-alcohol. To conclude, our findings suggest that both target-site and metabolic mechanisms underlie bifenthrin resistance in T. urticae, and these might synergize high levels of resistance.

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Section: Discussionmentioning

confidence: 96%

High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

Beer

Vandenhole

Njiru

et al. 2022

Biology

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Ácaros asociados al maíz en México

Chaires-Grijalva

Serrano-Domínguez

Coronado-Blanco

2021

Remexca

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Para México, el maíz (Zea mays L.) es uno de los cultivos con mayor importancia cultural y económica. Uno de los problemas que disminuyen su producción es la presencia de plagas primarias, que al ser controladas con insecticidas químicos contribuyen a la aparición de otras consideradas secundarias, como los ácaros. En el cultivo de maíz se han registrado nueve especies de ácaros que pertenecen a las familias Acaridae y Tetranychidae y una especie sin identificar de Iolinidae. Las especies con un amplio registro de distribución pertenecen a los géneros Oligonychus y Tetranychus, pertenecientes a la familia Tetranychidae, principalmente de hábitos fitófagos. En plantas infestadas se observa un aspecto blancuzco o bronceado del follaje. Las poblaciones económicamente importantes aparecen durante junio, julio y agosto, particularmente si el clima es cálido y seco. La mayor afectación al rendimiento se presenta cuando los ácaros dañan las hojas en o arriba del nivel de la mazorca. Las etapas del cultivo donde se cuantifican más daños son al inicio de la floración y llenado de grano, ya que la presencia de altas poblaciones de ácaros en estas etapas provoca que se produzcan mazorcas raquíticas con granos pequeños debido a la deshidratación que causan. Como estrategias de manejo se pueden utilizar las prácticas culturales, mejoramiento del cultivo, enemigos naturales y como último recurso los acaricidas. Por lo anterior, esta revisión de especies de ácaros que son considerados como plaga en uno de los cultivos más importantes de México, tiene como objetivo exponer diferentes aspectos biológicos y ecológicos importantes en la interacción ácaro-maíz.

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Molecular genetic analysis of resistance-associated mutations in the experimental lines of spider miteTetranychus urticaeKoch, selected for resistance to bifenthrin and abamectin

Cited by 2 publications

References 44 publications

High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

Ácaros asociados al maíz en México

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