No abstract

Mice were exposed to aerosolized ovalbumin (OA) once weekly for 5 min on 6 occasions, prior to systemic challenge with soluble or alum-adsorbed (AH-OA) antigen. Mice challenged with AH-OA manifested profound IgE isotype-specific tolerance; those challenged with soluble OA initially manifested anamnestic IgE/IgG responses, but secondary intraperitoneal immunization 13 days later with soluble OA revealed IgE isotype-specific tolerance. The tolerized mice contained splenic suppressor T cells which inhibited IgE but not IgG responses in an adoptive transfer assay. Tolerance was still demonstrable in mice 6 months after the cessation of aerosol exposures. Exposure of low IgE responder rat strains to aerosolized OA tolerized for both IgE and IgG responses.

CHD-FA is a promising topical remedy for drug-resistant wound infections. It accelerated the healing process of wounds infected with methicillin-resistant S. aureus and multidrug-resistant P. aeruginosa in rats, which is linked to both its antimicrobial and anti-inflammatory properties.

Spleen cells were prepared from Balb/c mice immunized 30 days previously with alum-precipitated ovalbumin (OA), which manifested high, persistent titres of anti-OA IgE and IgG. The adoptive transfer of 5.0 × 10⁷ such cells to X-irradiated syngeneic recipients produced comparable persistent IgE/IgG responses, in the absence of secondary antigenic challenge. Fractionation procedures indicated that the nylon-wool adherent population from the spleen was the most active in effecting transfer of the response. However, donor T-cells were also required, as pretreatment of the cellular inoculum with anti-Thy 1.2 antiserum ablated transfer. The inclusion of serum containing anti-OA IgG (but not IgE) in the cellular inoculum also blocked the transfer.