Adoptive Transfer of ‘Persistent’ IgE Responses in Mice in the Absence of Secondary Antigenic Stimulation

Abstract

Spleen cells were prepared from Balb/c mice immunized 30 days previously with alum-precipitated ovalbumin (OA), which manifested high, persistent titres of anti-OA IgE and IgG. The adoptive transfer of 5.0 × 10⁷ such cells to X-irradiated syngeneic recipients produced comparable persistent IgE/IgG responses, in the absence of secondary antigenic challenge. Fractionation procedures indicated that the nylon-wool adherent population from the spleen was the most active in effecting transfer of the response. However, donor T-cells were also required, as pretreatment of the cellular inoculum with anti-Thy 1.2 antiserum ablated transfer. The inclusion of serum containing anti-OA IgG (but not IgE) in the cellular inoculum also blocked the transfer.

Immunogenicity and Tolerogenicity of a Major House Dust Mite Allergen, <i>Der p</i> l from <i>Dermatophagoides pteronyssinus</i>, in Mice and Rats

“…We present evidence which indi cates that the purified allergen is tolerogenic in ani mals exposed to this allergen via a mucosal route, in particular the respiratory system. These data are con sistent with those obtained using the experimental an tigen ovalbumin reported previously from our labora tory [6][7][8],…”

Tolerance Induction via Antigen Inhalation: Isotype Specificity, Stability, and Involvement of Suppressor T Cells

“…In this system, persistent high titre IgE/IgG responses induced by alum-adsorbed anti gen in high-responder mice, may be transferred to Xirradiated recipients via the intravenous inoculation of splenocytes from the animals 30 days after initial donor priming. As shown here in a recent study [5], when used to transfer on-going anti-OA responses in the Balb/C strain, this system yields IgE/IgG re- . AH-OA-primed mice, exposed to aerosol (circles) or unexposed controls (triangles); soluble OA-primed mice, exposed to aerosol (squares) or unexposed con trols (stars).…”

“…sponses in recipients of comparable (or greater) mag nitude to the original donors, and without the necessi ty of further antigenic stimulation. Furthermore, such responses are highly sensitive to suppressor cells [5], and hence provide a convenient assay system for sup pressor cell activity. Employing this system, the pres ence of splenocytes from aerosolized mice in the cel lular inoculum abrogates adoptive transfer of a con siderable proportion of the IgE response, leaving the IgG response intact.…”

Antibody synthesis by bone marrow cells in vitro following primary and booster tetanus toxoid immunization in humans.

“…Alternatively, IgM-Tet-synthesizing lymphoblastoid cells could arise in the bone marrow without a prior blood-borne phase as the bone marrow provides the major environment for B cell development. Studies in rodents suggest that under some circumstances it is possible to transfer immune memory from donor to recipient by the transplantation of immune spleen cells (24). In other circumstances, immune memory is lost or impaired after adoptive transfer.…”