This study aims to assess the genetic diversity and population structure of two major zebu dairy breeds (Tharparkar and Rathi) adapted to the arid region of Rajasthan state of India. Various variability estimates indicate the existence of sufficient within-breed genetic diversity. Mean estimates of F-statistics are significantly different from zero: F (IS) = 0.112 +/- 0.029, F (IT) = 0.169 +/- 0.033, F (ST) = 0.065 +/- 0.017. The overall positive value of F (IS) (0.112) and an F (IT) value (0.169) that is more than the F (ST) (0.065) indicate departure from random mating. The drift-based estimates reflect a moderate yet significant level of breed differentiation between the Tharparkar and Rathi breeds. The evaluation of an exact test, showing that allele frequencies across all the loci differed significantly, supports the population differentiation. This is paralleled by the outcome of neighbor-joining clustering based on allele-sharing distance measures. The allocation of a high percentage of individuals (95.7%) to their population of origin and correspondence analysis further substantiates the existence of a cohesive genetic structure in both the breeds.
Protocols have been developed for the in vitro production of plants from callus derived from root explants of Lathyrus sativus cv. P-24, Callus and shoot regeneration were achieved only in MS medium supplemented with 10.7 p,M naphthaleneacetic acid and an increased concentration of kinetin (0.9 ~M for 14 days to 1.4 ~M for 18 days) during callusing. The shoots obtained rooted in 1/2MS supplemented with 0.5 p~M indolebutyric acid. During the year plants have been regenerated several times, The requirement for growth regulators is very specific and narrow.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.