In vitro plant regeneration from callus derived from root explants of Lathyrus sativus

Abstract

Protocols have been developed for the in vitro production of plants from callus derived from root explants of Lathyrus sativus cv. P-24, Callus and shoot regeneration were achieved only in MS medium supplemented with 10.7 p,M naphthaleneacetic acid and an increased concentration of kinetin (0.9 ~M for 14 days to 1.4 ~M for 18 days) during callusing. The shoots obtained rooted in 1/2MS supplemented with 0.5 p~M indolebutyric acid. During the year plants have been regenerated several times, The requirement for growth regulators is very specific and narrow.

In VitroPlant Regeneration from Turkish Grasspea (Lathyrus SativusL.) Using Immature Zygotic Embryo Explant

“…Shoot regeneration from immature zygotic embryos has also been described by Sahin-Demirbag et al (14) in L. cicera. This is in contrast to the results of (12) found that callus formation and shoot regeneration were achieved only in MS medium supplemented with 10.7 µM naphthalene acetic acid and 0.9 -1.4 µM kinetin.…”

“…This is in contrast to the observations of Barik et al (1, 2); who found it necessary to root the regenerating shoots in half strength (1/2) MS medium supplemented with iAA, iBA, or iPA and were able to elongate the roots only after the rooted shoots were transferred to half strength MS lacking the auxin. Roy et al (12) also rooted the shoots of L. sativus in 1/2 strength MS medium supplemented with 0.5µM indole 3 butyric acid. No root formation was observed when shoots were cultured in the medium devoid of auxins in agreement with Barik et al (1,2).…”

Callus Induction and Rhizogenesis in Lathyrus sativus L.

“…Tissue formed on the light was usually green colour, which obviously resulted from the chlorophyll synthesized under such conditions. Induction of callus from various grass pea explants and further tissue growth was carried out on the light (Barik and Kar, 2005;Roy et al, 1991Roy et al, , 1992Roy et al, , 1993Sinha et al, 1983) or in the dark for 2 to 7 days, and then on the light Maheshwari, 1980, 1983;Zambre et al, ). Just as the callus induction, its proliferation proceeded without major problems.…”

“…Barik and Kar (2005) noted the best rate of grass pea callus formation on the MS medium supplemented in 3.0 mg/l NAA and 0.3 mg/l BAP. Such combination of growth regulators was also successfully applied in other callus culture of L. sativus (Barik and Kar, 2005;Barik et al, 2006;Roy et al, 1993;Roy et al, 1991;Sinha et al, 1982). However, in our studies callus proliferation was the most intense on the medium containing 0.5 mg/l NAA and 1.0 mg/l TDZ.…”

“…Earlier studies showed that apart from the diffi culties in the regeneration of shoots from callus tissue of grass pea (Gharyal and Maheshwari, 1983;Sinha et al, 1983) it is very diffi cult to get the whole plant by indirect organogenesis and it succeeded only in a few cases in this species (Barik and Kar, 2005;Roy et al, 1991;Roy et al, 1992;Zambre et al, 2002). Furthermore, in all reports on Lathyrus sativus, successful plant regeneration was very genotypedependent.…”