Many bacteria secrete a highly hydrated framework of extracellular polymer matrix on suitable substrates and embed within the matrix to form a biofilm. Bacterial biofilms are observed on many medical devices, endocarditis, periodontitis and lung infections in cystic fibrosis patients. Bacteria in biofilm are protected from antibiotics and >1,000 times of the minimum inhibitory concentration may be required to treat biofilm infections. Here, we demonstrated that shock waves could be used to remove Salmonella, Pseudomonas and Staphylococcus biofilms in urinary catheters. The studies were extended to a Pseudomonas chronic pneumonia lung infection and Staphylococcus skin suture infection model in mice. The biofilm infections in mice, treated with shock waves became susceptible to antibiotics, unlike untreated biofilms. Mice exposed to shock waves responded to ciprofloxacin treatment, while ciprofloxacin alone was ineffective in treating the infection. These results demonstrate for the first time that, shock waves, combined with antibiotic treatment can be used to treat biofilm infection on medical devices as well as in situ infections.

A novel concept to generate miniature shockwaves in a safe, repeatable, and controllable manner in laboratory confinements using an in situ oxyhydrogen generator has been proposed and demonstrated. This method proves to be more advantageous than existing methods because there is flexibility to vary strength of the shockwave, there is no need for storage of high pressure gases, and there is minimal waste disposal. The required amount of oxyhydrogen mixture is generated using alkaline electrolysis that produces hydrogen and oxygen gases in stoichiometric quantity. The rate of oxyhydrogen mixture production for the newly designed oxyhydrogen generator is found to be around 8 ml/s experimentally. The oxyhydrogen generator is connected to the driver section of a specially designed 10 mm square miniature shock tube assembly. A numerical code that uses CANTERA software package is used to predict the properties of the driver gas in the miniature shock tube. This prediction along with the 1-D shock tube theory is used to calculate the properties of the generated shockwave and matches reasonably well with the experimentally obtained values for oxyhydrogen mixture fill pressures less than 2.5 bars. The miniature shock tube employs a modified tri-clover clamp assembly to facilitate quick changing of diaphragm and replaces the more cumbersome nut and bolt system of fastening components. The versatile nature of oxyhydrogen detonation-driven miniature shock tube opens up new horizons for shockwave-assisted interdisciplinary applications.

No abstract

Despite extensive research on developing different transition metal boride composites for aero-thermostructural applications, the understanding of the shockwave interaction using high pressure shock testing facilities and computational simulation of such interactions are much less explored. This aspect is even more important for much less explored ceramics, like NbB 2 -based materials. While addressing this aspect, the present investigation reports the thermostructural stability of spark plasma sintered NbB 2 -(0-40) mol % B 4 C composites under the hypersonic aerothermodynamic conditions using a miniature detonation-driven shock tube facility. All the ceramic discs underwent mild surface oxidation, as a consequence to impulsive load together with the thermomechanical shock. Using the in situ recorded pressure pulse data together with conjugate heat transfer analysis, spatiotemporal evolution of ceramic surface temperature was computationally analyzed for the given test conditions. Importantly, the NbB 2 -(0 and 20) mol % B 4 C composite retained structural integrity even after exposure to 10 shock pulses with maximum reflected shock temperature and pressure of 5000 K and 37.5 MPa, respectively. In contrast, NbB 2 -40 mol % B 4 C underwent structural failure by shattering to pieces. An attempt has been made to rationalize such results on the basis of thermal shock resistance parameters, estimated using the Kingery and Hasselman model. It is observed that NbB 2 -(0 and 20) mol % B 4 C shows higher crack propagation resistance, that is, 20 and 30%, respectively, under thermal shock (R″) than NbB 2 -40 mol % B 4 C. Interestingly, all the shock exposed NbB 2 -B 4 C ceramics show a measurable increase in hardness, which is attributed to transient melting and solidification of constituent phases due to interaction with shock heated gas, for a short duration of ∼5 ms. Taken together, the present study establishes the potential of NbB 2 -B 4 C composites for aero-thermostructural applications.

No abstract

No abstract

Abstract

Scientific Reports 5: Article number: 17440; published online: 10 December 2015; updated: 08 January 2018 Figure S6 in the Supplementary Information contains errors. Due to misfiling of data the figure was prepared incorrectly. The image in figure S6g corresponds to the incorrect experimental group:‘Mice infected with P.