The total flavonoid content of the flowers, fruits, leaves, and small branches of Sophora japonica L. was determined colorimetrically after addition of 0.lM aqueous aluminum chloride. The total flavonoid and isoflavonoid content of the fruits was determined gravimetrically. Rutin was separated by thin layer chromatography from the different organs, and determined either densitometrically or spectrophotometrically, using a modified elution technique. Rutin was recovered almost completely with an average loss of 2.5%. The percentages of flavonoids and rutin of the flower buds, pericarps, seeds, leaflets, and small branches were 24.29 and 23.5, 10.57 and 4.3, 1.75 and 0.55, 6.63 and 4.7, and 1.5 and 0.26, respectively, while the total flavonoid and isoflavonoid content of the fruits was 43%.
ESTIMATION O F CARDENOLIDES IN NERlUM OLEANDER-B y M. S. K a r a w y a , S. I. B a l b a a and S. E. K h a y y a l Recently, K a r a w y a et al; (1969) made use of Baljet's reagent in the estimation of total cardenolidal content of certain organs of Neriunz oleander L., and reported that the egyptian plants contained about 0.8% of cardiac glycosides calculated as oleandrin. S t r a u b (1918), however reported that the leaves contained about 1.15% of oleandrin, while W o l f r e d (1949), mentioned that the yield was 0.08%. G d r l i c h (1962), on the other hand, stated that the leaves obtained from plants cultivated in Italy contained 0.55% of cardiac glycosides. Owing to the above controversy in the cardenolidal content of Nerium oleander, this work was planned to throw light on oleandrin and adynerin contents of oleander plants producing white o r red flowers, employing a TLC-colorimetric method.
Plants containing mucilage have been, and are still, the subject of vast investigations because of their importance as medicinal and pharmaceutical materials which are commonly used as drugs as well as in many food and textile industries. Among the drugs containing mucilage are the seeds of Plantago psyllium L., the roots, leaves and flowers of Malva sylvestris L., the roots and leaves of Althaea officinalis L., as well as the roots of Glossostemon brziguieri D e s f. (moghat).
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