The total flavonoid content of the flowers, fruits, leaves, and small branches of Sophora japonica L. was determined colorimetrically after addition of 0.lM aqueous aluminum chloride. The total flavonoid and isoflavonoid content of the fruits was determined gravimetrically. Rutin was separated by thin layer chromatography from the different organs, and determined either densitometrically or spectrophotometrically, using a modified elution technique. Rutin was recovered almost completely with an average loss of 2.5%. The percentages of flavonoids and rutin of the flower buds, pericarps, seeds, leaflets, and small branches were 24.29 and 23.5, 10.57 and 4.3, 1.75 and 0.55, 6.63 and 4.7, and 1.5 and 0.26, respectively, while the total flavonoid and isoflavonoid content of the fruits was 43%.
The total alkaloids of lobelia herb and its tincture were estimated colorimetrically after reaction with formaldehyde-sulfuric acid reagent; the results were found to be comparable with those of the 1963 Egyptian Pharmacopoeia method. The alkaloids lobeline, lobelanine, and lobelanidine were estimated individually in the herb and tincture after using the TLC-colorimetric method. The method was found to be sensitive and applicable to pharmaceutical preparations such as lobeline sulfate tablets and lobeline hydrochloride ampuls.
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