ESTIMATION O F CARDENOLIDES IN NERlUM OLEANDER-B y M. S. K a r a w y a , S. I. B a l b a a and S. E. K h a y y a l Recently, K a r a w y a et al; (1969) made use of Baljet's reagent in the estimation of total cardenolidal content of certain organs of Neriunz oleander L., and reported that the egyptian plants contained about 0.8% of cardiac glycosides calculated as oleandrin. S t r a u b (1918), however reported that the leaves contained about 1.15% of oleandrin, while W o l f r e d (1949), mentioned that the yield was 0.08%. G d r l i c h (1962), on the other hand, stated that the leaves obtained from plants cultivated in Italy contained 0.55% of cardiac glycosides. Owing to the above controversy in the cardenolidal content of Nerium oleander, this work was planned to throw light on oleandrin and adynerin contents of oleander plants producing white o r red flowers, employing a TLC-colorimetric method.
Red squill bulbs, with reported specific rodenticidal properties, have been assayed for their content of the two main glycosides, scilliroside and scillaren A, by a method depending on the separation of the glycosides from purified plant extracts by tlc followed by spectrophotometric (uv and visible) determination of the individual glycosides in the eluates. The method was found convenient for assessment of the potency of red squill bulbs.
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