Determination of reserpine in pharmaceutical formulations by gas liquid chromatography

Khayyal, S.E.; Ayad, Magda M.; Girgis, A. N.

doi:10.1016/s0021-9673(01)87791-8

Cited by 5 publications

(3 citation statements)

References 2 publications

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“…Therefore, simultaneous determination of the analytes in biological samples is important in studying pharmacokinetics. However, to our best knowledge, no analytical method has been described in the literature which simultaneously determines these three analytes in biological samples, even though several methods exist, which separately determine either triamterene [1][2][3][4][5], hydrochlorothiazide [6][7][8], reserpine [9][10][11][12][13], or determine triamterene and hydrochlorothiazide simultaneously [14,15]. Though many kinds of analytical methods such as spectrophotometry, spectrofluorimetric, potentiometry, capillary electrophoresis, HPLC-UV, LC-MS were used in these literatures, it is tedious to determine three analytes separately for each sample using two different HPLC conditions or even two different detection methods.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

et al. 2016

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“…Therefore, simultaneous determination of the analytes in biological samples is important in studying pharmacokinetics. However, to our best knowledge, no analytical method has been described in the literature which simultaneously determines these three analytes in biological samples, although several methods exist which separately determine either triamterene 1–7, hydrochlorothiazide 8–12, reserpine 13–19 or determine triamterene and hydrochlorothiazide simultaneously 20, 21. Though many kinds of analytical methods such as spectrophotometry, spectrofluorimetric, potentiometry, capillary electrophoresis, HPLC‐UV and LC‐MS were used in these literatures, it is tedious to determine three analytes separately for each sample using two different HPLC conditions or even two different detection methods.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of hydrochlorothiazide, triamterene and reserpine in rat plasma by high performance liquid chromatography and tandem solid‐phase extraction

Li¹,

He²,

Q³

et al. 2011

J of Separation Science

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A tandem solid-phase extraction method (SPE) of connecting two different cartridges (C(18) and MCX) in series was developed as the extraction procedure in this article, which provided better extraction yields (>86%) for all analytes and more appropriate sample purification from endogenous interference materials compared with a single cartridge. Analyte separation was achieved on a C(18) reversed-phase column at the wavelength of 265 nm by high-performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89% with RSD values that were always less than 3.8%. The method has been successfully applied to plasma samples from rats after oral administration of target compounds.

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“…Analytical methods for hydrochlorothiazide individually or combined with other antihypertensive drugs in tablets or urine have been reported based on capillary electrophoresis method [8], high performance liquid chromatography (LC) with UV, electrochemical detection [6] or mass spectrometry (LC-MS), and liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) [2–8]. A variety of methods also have been applied for assaying reserpine, such as spectrofluorimetric [9–12], GC and HPLC methods with electrochemical detectors [13, 14], and LC/MS/MS with a chelating agent [15]. Most of these methods required specific detectors, such as an electrochemical detector [14], while others required complicated sample pre-treatment [15, 16].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Hydrochlorothiazide and Reserpine in Human Urine by LC with a Simple Pre-Treatment

Liu

et al. 2011

Chromatographia

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A simple, selective and sensitive reversed-phase high performance liquid chromatography method for simultaneous analysis of hydrochlorothiazide and reserpine in human urine was developed and subjected to primary pharmacokinetic study. After a simple protein precipitation using methanol and extraction with ethyl acetate, the analytes were separated on an Elite C 18 column at a flow rate of 0.8 mL min −1 . The mobile phase was composed of acetonitrile (A) and 0.2% ammonium chloride solution (B) for a gradient elution starting at A:B at 30:70, v/v for 0~6 min, linearly raising the percent of A from 30% to 50% (6~9 min) and ending at 50:50, v/v (9~25 min). The standard curves were linear over the range of 0.05-20 µg mL −1 for hydrochlorothiazide and 0.02-5.0 µg mL −1 for reserpine, respectively (r > 0.999). The limit of detection (LOD) and the limit of quantification (LOQ) were 5.5 ng mL −1 and 18.2 ng mL −1 for hydrochlorothiazide, and 7.1 ng mL −1 and 23.6 ng mL −1 for reserpine, respectively. The recoveries for both analytes were above 89.0±1.35%. The intra-day and inter-day precision for hydrochlorothiazide were less than 1.91% and 1.38%, and those for reserpine were below 1.61% and 2.64%, respectively. The method indicated good performance in terms of specificity, linearity, detection and quantification limits, precision and accuracy, and it was employed successfully for the simultaneous determination of hydrochlorothiazide and reserpine in human urine samples.

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Determination of reserpine in pharmaceutical formulations by gas liquid chromatography

Cited by 5 publications

References 2 publications

Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

Simultaneous analysis of hydrochlorothiazide, triamterene and reserpine in rat plasma by high performance liquid chromatography and tandem solid‐phase extraction

Simultaneous Determination of Hydrochlorothiazide and Reserpine in Human Urine by LC with a Simple Pre-Treatment

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