This study evaluated the potential of the PCL (poly -caprolactone)/HA(Hydroxyapatite) composite materials as a scaffold for bone regeneration. For this, we fabricated scaffolds utilizing salt leaching method. The PCL/HA composite scaffolds were prepared with various HA contents (20wt%, 40wt%, 60 wt %). To ensure the potential for the scaffolds, porosity tests were conducted along with SEM observations. The porosity decreased with the increase of the contents of HA particles. The porosity of the composite with the highest contents of HA was still adoptable (~85%). In addition, the PCL/HA composite scaffolds were evaluated for their ability of osteogenic differentiation with human bone marrow stromal cell (hBMSC) in vitro. Alkaline phosphatase (ALP) activity, markers for osteoblastic differentiation, and total protein contents were evaluated in hBMSCs following 14 days of cultivation. The addition of HA particles enhanced proliferation of hBMSC during the test. Also, the differentiation ability of the cells was increased as HA particles were added. In this study, we concluded that PCL/HA composite scaffolds has great potential as a scaffold for bone tissue engineering.
PMMA remains the most popular material of bone cement for orthopaedic surgeries.
However, conventional PMMA bone cement still has some problems. For this, we suggested new
composite material (BBC) consisting of hydroxyapatite (45%), chitosan (10%) and PMMA. The
purpose of this study was to evaluate the bonding stress at the interface of PMMA with additives
and host bone using a rabbit model. After 6, 12 weeks of operation, the bonding stresses were
evaluated by measuring shear stress through push-out test. The results of the tests showed that after
6 weeks the shear stress of the BBC was 2.65±0.29MPa and the PMMA was 1.21±0.31MPa
(p<0.05). However, after 12 weeks, there were no significant differences between BBC and
conventional PMMA bone cement. In SEM analysis, bone surface of BBC showed higher
roughness than that of conventional PMMA bone cement after push-out test. From the study we
conducted, addition of HA particles and chitosan to conventional PMMA bone cement showed
promising results. The BBC has clinical potential of bone substitutes replacing conventional
PMMA.
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