In Vitro Evaluation of Poly ε-Caprolactone/Hydroxyapatite Composite as Scaffolds for Bone Tissue Engineering with Human Bone Marrow Stromal Cells

Heo, Su Jin; Kim, S.E.; Hyun, Yong-Taek; Kim, D.H.; Lee, Hyang Mi; Hwang, Yeong‐Maw; Park, S.A.; Shin, Jung Woog

doi:10.4028/www.scientific.net/kem.342-343.369

Cited by 12 publications

(4 citation statements)

References 12 publications

(10 reference statements)

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“…However, the high hydrophobicity of PCL relative to that of natural extracellular matrix (ECM) leads to poor cell behavior [3]. Some studies have demonstrated that adding bioactive materials, such as hydroxyapatite (HAp), [4] tricalcium phosphate [5], and bioactive glass [6], to PCL can improve the hydrophilicity of PCL. Lee et al showed that the incorporation of bioactive glass can significantly enhance cell activities on PCL membranes, indicating favorable osteoconductivity and osteoinductivity for GBR [7].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characteristics of PCL Membranes Containing Strontium-Substituted Hydroxyapatite Nanofibers for Guided Bone Regeneration

Tsai

Hwang

et al. 2019

Polymers

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Poly(ε-caprolactone) (PCL) membranes have been widely used in guided tissue regeneration (GTR) and guided bone regeneration (GBR). In addition, hydroxyapatite is the major inorganic component and an essential composition of hard bone and teeth. Recently, numerous studies have demonstrated that strontium-substituted hydroxyapatite (SrHA) not only enhances osteogenesis but also inhibits adipogenesis of mesenchymal stem cells. Therefore, SrHA incorporated into PCL could be an alternative material for GBR. In this study, strontium-substituted hydroxyapatite nanofibers (SrHANFs) were fabricated by a sol–gel route followed by electrospinning. We then fabricated PCL–SrHANF membranes as cell culture substrates and assessed the cellular behavior of osteoblast-like cells. Based on the observations of alkaline phosphatase (ALP) activity, bone sialoprotein (BSP) and osteocalcin (OCN) immunofluorescence staining, and Alizarin Red-S staining of cells cultured on the PCL–SrHANF and PCL membranes, we concluded that SrHANFs can promote the differentiation and mineralization of osteoblast-like cells and that PCL–SrHANF membranes have potential for GBR applications.

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Section: Introductionmentioning

confidence: 99%

Fabrication and Characteristics of PCL Membranes Containing Strontium-Substituted Hydroxyapatite Nanofibers for Guided Bone Regeneration

Tsai

Hwang

et al. 2019

Polymers

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“…Figure 5 showed the XRD spectra of different ratios of PCL/COL vascular grafts. It could be seen from the figure that there were two obvious peaks at 21.3 • and 23.8 • [30] for different ratios of grafts. This was the diffraction peak of PCL, and the corresponding crystal plane indices were (110) and (200), respectively.…”

Section: Xrd Analysismentioning

confidence: 92%

Construction of PCL-collagen@PCL@PCL-gelatin three-layer small diameter artificial vascular grafts by electrospinning

Lu¹,

Zou²,

Liao³

et al. 2022

Biomed. Mater.

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The demand for artificial vascular grafts in clinical applications is increasing, and it is urgent to design a tissue-engineered vascular graft with good biocompatibility and sufficient mechanical strength. In this study, three-layer small diameter artificial vascular grafts were constructed by electrospinning. Polycaprolactone (PCL) and collagen (COL) were used as the inner layer to provide good biocompatibility and cell adhesion, the middle layer was PCL to improve the mechanical properties, and gelatin (GEL) and PCL were used to construct the outer layer for further improving the mechanical properties and biocompatibility of the vascular grafts in the human body environment. The electrospun artificial vascular graft had good biocompatibility and mechanical properties. Its longitudinal maximum stress reached 2.63±0.12MPa, which exceeded the maximum stress that many natural blood vessels could withstand. The fibre diameter of the vascular grafts was related to the proportion of components that made up the vascular grafts. In the inner structure of the vascular grafts, the hydrophilicity of the vascular grafts was enhanced by the addition of COL to the PCL, and Human umbilical vein endothelial cells (HUVECs) adhered more easily to the vascular grafts. In particular, the cytocompatibility and proliferation of HUVECs on the scaffold with an inner structure PCL:COL = 2:1 was superior to other ratios of vascular grafts. The vascular grafts didn’t cause hemolysis of red blood cells. Thus, the bionic PCL-COL@PCL@PCL-GEL composite graft is a promising material for vascular tissue engineering.

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“…Additionally, an open and fully interconnected porosity is needed to achieve full porogen removal. In the literature [ 33 , 34 , 35 , 36 , 37 , 38 ], many examples are available related to the fabrication of PCL/CaP composite scaffolds using the SC/PL technique and conversely, a very limited number of studies address the addition of antibacterial agents. A few previous studies have investigated the role of antimicrobial agents, such as copper oxide [ 39 ], ZnO [ 40 ], and β-silver vanadate oxide (AgVO) 3 [ 41 ] particles, in non-porous solvent cast films.…”

Section: Development Processes Of Pcl-based Biomaterialsmentioning

confidence: 99%

New Generation of Osteoinductive and Antimicrobial Polycaprolactone-Based Scaffolds in Bone Tissue Engineering: A Review

Coppola,

Menotti,

Longo

et al. 2024

Polymers

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With respect to other fields, bone tissue engineering has significantly expanded in recent years, leading not only to relevant advances in biomedical applications but also to innovative perspectives. Polycaprolactone (PCL), produced in the beginning of the 1930s, is a biocompatible and biodegradable polymer. Due to its mechanical and physicochemical features, as well as being easily shapeable, PCL-based constructs can be produced with different shapes and degradation kinetics. Moreover, due to various development processes, PCL can be made as 3D scaffolds or fibres for bone tissue regeneration applications. This outstanding biopolymer is versatile because it can be modified by adding agents with antimicrobial properties, not only antibiotics/antifungals, but also metal ions or natural compounds. In addition, to ameliorate its osteoproliferative features, it can be blended with calcium phosphates. This review is an overview of the current state of our recent investigation into PCL modifications designed to impair microbial adhesive capability and, in parallel, to allow eukaryotic cell viability and integration, in comparison with previous reviews and excellent research papers. Our recent results demonstrated that the developed 3D constructs had a high interconnected porosity, and the addition of biphasic calcium phosphate improved human cell attachment and proliferation. The incorporation of alternative antimicrobials—for instance, silver and essential oils—at tuneable concentrations counteracted microbial growth and biofilm formation, without affecting eukaryotic cells’ viability. Notably, this challenging research area needs the multidisciplinary work of material scientists, biologists, and orthopaedic surgeons to determine the most suitable modifications on biomaterials to design favourable 3D scaffolds based on PCL for the targeted healing of damaged bone tissue.

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In Vitro Evaluation of Poly ε-Caprolactone/Hydroxyapatite Composite as Scaffolds for Bone Tissue Engineering with Human Bone Marrow Stromal Cells

Cited by 12 publications

References 12 publications

Fabrication and Characteristics of PCL Membranes Containing Strontium-Substituted Hydroxyapatite Nanofibers for Guided Bone Regeneration

Fabrication and Characteristics of PCL Membranes Containing Strontium-Substituted Hydroxyapatite Nanofibers for Guided Bone Regeneration

Construction of PCL-collagen@PCL@PCL-gelatin three-layer small diameter artificial vascular grafts by electrospinning

New Generation of Osteoinductive and Antimicrobial Polycaprolactone-Based Scaffolds in Bone Tissue Engineering: A Review

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