IgG2, G2m(23) anti-Rh-D antibodies were detected in the serum of a donor. It has been characterized by Gm typing and the use of polyclonal and mouse monoclonal anti-IgG subclasses (which had proved to be satisfactory for agglutination of sensitized red blood cells). This anti-D constitutes a good reagent for G2m(23) typing.
IgG2, G2m(23) anti-Rh-D antibodies were detected in the serum of a donor. It has been characterized by Gm
typing and the use of polyclonal and mouse monoclonal anti-IgG subclasses (which had proved to be satisfactory for
agglutinaton of sensitized red blood cells). This anti-D constitutes a good reagent for G2m(23) typing.
As already found in other various diseases, a macromolecular alkaline phosphatase complex (HMW-AP) was also found in sera of two severe Rhesus-incompatible pregnancies complicated by ascites and fetal hydrops at delivery. This atypical complex was detected and isolated by agarose gel electrophoresis. Immunoelectrophoresis and heat inactivation of this HMW-AP complex revealed that it consisted of IgG of the kappa type and placental AP isoenzyme. The transitory presence of this immuncomplex is discussed. However, in all women with Rh-immunized complicated pregnancies, significant variations of neutrophil and serum AP activities were observed. A fall in AP activity and the presence of an antiplacental AP antibody in serum of women with complicated Rh immunization should be of value in assessing the prognosis of the disease.
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