There have been increasing needs for biosensing in the food industry because it is accurate, rapid and efficient. A needle-type L-lactate sensor with a three-layer membrane system which uses lactate oxidase as the biological component was prepared. The sensor had an optimum pH around 9-10 and an optimum temperature at 45ЊC. The current response was stable over 40 days and specifically responded to L-lactic acid. The sensor gave accurate L-lactate measurements in kimchi and yogurt of 187.4 ע 4.1 and 734.1 ע 34.5 mg/dL, similar to those of a spectrophotometric L-lactate kit.
Cereal Chem. 74(6):745-749The determination of damaged starch and diastatic activity in flour was studied using a flow-injection analysis (FIA) biosensor system. The system consisted of an oxygen electrode and an immobilized enzyme column containing purified glucoamylase and glucose oxidase immobilized on activated aminopropyl glass beads. The biosensor system has an optimum pH between 6.5 and 7.5 and an optimum temperature of 35°C for glucose measurement. The response of the FIA biosensor was linear up to 1.000 g/L of glucose with a lower detection limit of 0.025 g/L. Each assay took about 20 min, and the system showed good reproducibility (r = 0.998, n = 8). When applied to the measurement of damaged starch and diastatic activity in wheat flour, the results obtained agreed with those obtained using the conventional methods of measurement. This biosensor system is a rapid practical alternative for the measurement of damaged starch and diastatic activity in wheat flour.
The ash content of wheat flour is used to evaluate its quality but the conventional method of analysis takes several hours. This paper proposes using the potassium ion concentration of wheat flour samples instead of the ash content since the correlation between the two is very good (the correlation coefficient was 0.99). A flow injection analysis (FIA) system based on a potassium ion selective electrode took less than 10min per measurement with a coefficient of variation less than 3%.
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