Characterization and Food Application of an Amperometric Needle‐Type L‐Lactate Sensor

Kim, Namsoo; Haginoya, Ryuichi; Karube, Isao

doi:10.1111/j.1365-2621.1996.tb14177.x

Cited by 16 publications

(6 citation statements)

References 26 publications

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“…Results from food sample measurement showed the system to be a simple, easily-handled instrument for quality control in the food industry. Subsequently, an amperometric needle-type L-lactate sensor was fabricated and applied to kimchi and yogurt samples [316]. This sensor had a three-layer membrane system that used lactate oxidase as the biological component.…”

Section: Food Components Sensorsmentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

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260

129

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Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks many biosensors and their associated techniques have been studied and developed. In this review current research activity on the fundamentals and applications of biosensors is summarized. In discussion of the former, molecular recognition elements, techniques and tools for biosensor construction, and basic biosensor devices are introduced. Coverage of the latter includes description of biosensors for environmental, food, and clinical fields. Chemical sensors developed in our laboratory for environmental monitoring are also described. This review mainly summarizes work performed by our group and by our colleagues, and refers to the main review articles summarizing each field of biosensor research in recent years.

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Section: Food Components Sensorsmentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

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260

129

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“…This phenomenon seemed to be attributable to efficient binding and orientation of the enzyme over the QCM surface (Bain et al, 1989). The CV value of 0.56% found for the QCM-precipitation sensor prepared by the former method was low enough to show a good performance, considering the normally accepted 5% level for a reasonable analytical method including biosensor technique (Kim et al, 1996).…”

Section: Ache Binding and Sensor Response According To Immobilizationmentioning

confidence: 98%

High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor

Kim

Park

Kim

2007

Biosensors and Bioelectronics

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“…Other approaches which have also been applied in the detection of L-lactate in dairy products involve entrapment of the biological recognition element in a nonconducting film such as poly (ophenylendiamine) and overoxidized polypyrrole (8), polypyrrole-polyvinylsulfonate composite films (9), and glutaraldehyde and overoxidized polypyrrole (10). Furthermore, there has been development within biosensor research to miniaturize the entire sensing system for application within the food industry using silicon sensor chip technology (11) and development of needletype sensors (12).…”

Section: Introductionmentioning

confidence: 99%

Amperometric Determination of l-Lactate Based on Entrapment of Lactate Oxidase on a Transducer Surface with a Semi-Permeable Membrane Using a SIRE Technology Based Biosensor. Application: Tomato Paste and Baby Food

Kriz

Kraft

Krook

et al. 2002

J. Agric. Food Chem.

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Determination of dissolved L-lactate in tomato paste and baby food samples using a SIRE-based (sensors based on injection of the recognition element) biosensor is reported. The measuring principle is based on the use of a small amount of enzyme, which is injected into an internal delivery flow system and held in direct spatial contact with the amperometric transducer by the use of a semipermeable membrane. Measurements are based upon the reversible enzymatic conversion of L-lactate to pyruvate and hydrogen peroxide by lactate oxidase. Differential measurements are performed in which the samples are measured in the presence and absence of enzyme allowing for control over matrix interferences present in crude samples. The linear range investigated for the determination of L-lactate in tomato paste and baby food was 0-0.1 mM using a lactate oxidase concentration of 22 U/mL. Samples were diluted with buffer prior to biosensor measurements. The L-lactate concentrations of the tomato paste and baby food were determined to be 1.02 +/- 0.02 mM and 2.51 +/- 0.10 mM, respectively, using the standard addition method. The repeatability for tomato paste and baby food measurements was 2.5% (RSD, n = 15) and 4.0% (RSD, n = 15) and the reproducibility was 13.0% (RSD, n = 45) and 3.0% (RSD, n = 45), respectively. The concentration of dissolved L-lactate can be used as a measure of freshness in the food industry. All biosensor measurements were compared with measurements from an established spectrophotometric assay (Boehringer Mannheim). It was found that the biosensor had good correlation with the spectrophotometric method. The biosensor gave 12% higher values for the tomato paste measurements and 2.5% higher values for the baby food measurements. However, a distinct advantage of the biosensor is that it can perform L-lactate measurements within 3 minutes, whereas the spectrophotometric assay requires a 35-minute measurement time.

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Characterization and Food Application of an Amperometric Needle‐Type L‐Lactate Sensor

Cited by 16 publications

References 26 publications

Current research activity in biosensors

Current research activity in biosensors

High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor

Amperometric Determination of l-Lactate Based on Entrapment of Lactate Oxidase on a Transducer Surface with a Semi-Permeable Membrane Using a SIRE Technology Based Biosensor. Application: Tomato Paste and Baby Food

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