High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor

Biosensors Applications in Agri-food Industry

“…The antigen-antibody reaction is detected by the oscillating piezoelectric crystal quartz that resonates at a fundamental frequency and has a linear behavior with respect to its surface mass density. The operation of piezoelectric resonators in liquid phases includes changes in the mass induced frequency and effects induced by the change in viscosity or density of the surrounding liquid layers (Kim et al, 2007;Fohlerová et al, 2007). There are also other potentially toxic substances for humans, such as contaminated residues present in water and soil, with a major impact on the environment accidentally reaching the food chain (Serna et al, 2009).…”

Mercaptobenzothiazole-on-Gold Organic Phase Biosensor Systems: 4. Effect of Organic Solvents on Organophosphate and Carbamate Pesticide Determination

“…mass spectrometry in GC-MS), high performance liquid chromatography (HPLC) and HPLC coupled with mass spectrometry (HPLC-MS) or UV detection (HPLC-UV). However, despite the precision and accuracy of these methods, analyses are restricted to laboratory facilities, are time-consuming and expensive due to its analytical cost, limiting the operation of these instruments to highly qualified laboratory personnel (Somerset et al, 2009;Valdés-Ramírez et al, 2008;Kim et al, 2007;Gong et al, 2009;Caetano and Machado, 2008). Therefore, the need to find rapid, simple and sensitive methods for the detection of organophosphate (OP) and carbamate (CM) pesticide compounds are growing.…”

“…This poses serious health effects for humans if water, processed food, fruits and vegetables with high concentrations of OPs and CMs are consumed. In children and infants it can lead to serious health effects such as as eye pain, abdominal pain, convulsions, respiratory failure, paralysis and even death (Kim et al, 2007;Liu et al, 2008;Sbai et al, 2007;Skladal et al, 1997). The toxicity of OPs and CMs are caused by their ability to bind irreversibly to the catalytic serine residue in acetylcholinesterase (AChE), which leads to inhibition of AChE that prevents nerve transmission by blocking the breakdown of the transmitter choline (Ch) (Somerset et al, 2009;Liu et al, 2008;Kim et al, 2007;Du et al, 2007;Kim et al, 2000).…”

“…In children and infants it can lead to serious health effects such as as eye pain, abdominal pain, convulsions, respiratory failure, paralysis and even death (Kim et al, 2007;Liu et al, 2008;Sbai et al, 2007;Skladal et al, 1997). The toxicity of OPs and CMs are caused by their ability to bind irreversibly to the catalytic serine residue in acetylcholinesterase (AChE), which leads to inhibition of AChE that prevents nerve transmission by blocking the breakdown of the transmitter choline (Ch) (Somerset et al, 2009;Liu et al, 2008;Kim et al, 2007;Du et al, 2007;Kim et al, 2000). Since a high degree of toxicity is assigned to organophosphate (OP) and carbamate (CM) pesticide compounds, the rapid detection of these toxic chemicals in environmental samples have become increasingly important (Sbai et al, 2007;Tapsoba et al, 2009;Valdés-Ramírez et al, 2008).…”

Sistema Oscilador Mejorado para Aplicaciones de Microbalanza (QCM) en Medios Líquidos y Propuesta de un Nuevo Método de Caracterización para Biosensores Piezoeléctricos

“…El biosensor basado en QCM desarrollado por N. Kim y colaboradores [Namsoo Kim et al 2007] para la detección de carbamatos ofrece un LOD=0,26μg/l. Esta mayor sensibilidad no se debe al método de caracterización sino a la bioquímica asociada al biosensor; este trabajo se basa en la inhibición de la acetilcolinesterasa y no en inmunoensayos como en nuestro caso.…”