Spring wheat (Triticum aestivum L. cv. TRISO) was grown for three consecutive seasons in a free‐air carbon dioxide (CO2) enrichment (FACE) field experiment in order to examine the effects on crop yield and grain quality. CO2 enrichment promoted aboveground biomass (+11.8%) and grain yield (+10.4%). However, adverse effects were predominantly observed on wholegrain quality characteristics. Although the thousand‐grain weight remained unchanged, size distribution was significantly shifted towards smaller grains, which may directly relate to lower market value. Total grain protein concentration decreased significantly by 7.4% under elevated CO2, and protein and amino acid composition were altered. Corresponding to the decline in grain protein concentration, CO2 enrichment resulted in an overall decrease in amino acid concentrations, with greater reductions in non‐essential than essential amino acids. Minerals such as potassium, molybdenum and lead increased, while manganese, iron, cadmium and silicon decreased, suggesting that adjustments of agricultural practices may be required to retain current grain quality standards. The concentration of fructose and fructan, as well as amounts per area of total and individual non‐structural carbohydrates, except for starch, significantly increased in the grain. The same holds true for the amount of lipids. With regard to mixing and rheological properties of the flour, a significant increase in gluten resistance under elevated CO2 was observed. CO2 enrichment obviously affected grain quality characteristics that are important for consumer nutrition and health, and for industrial processing and marketing, which have to date received little attention.

The productivity, product quality and competitive ability of important agricultural and horticultural plants in many regions of the world may be adversely affected by current and anticipated concentrations of groundlevel ozone (O 3 ). Exposure to elevated O 3 typically results in suppressed photosynthesis, accelerated senescence, decreased growth and lower yields. Various approaches used to evaluate O 3 effects generally concur that current yield losses range from 5% to 15% among sensitive plants. There is, however, considerable genetic variability in plant responses to O 3 . To illustrate this, we show that ambient O 3 concentrations in the eastern United States cause substantially different levels of damage to otherwise similar snap bean cultivars. Largely undesirable effects of O 3 can also occur in seed and fruit chemistry as well as in forage nutritive value, with consequences for animal production. Ozone may alter herbicide efficacy and foster establishment of some invasive species. We conclude that current and projected levels of O 3 in many regions worldwide are toxic to sensitive plants of agricultural and horticultural significance. Plant breeding that incorporates O 3 sensitivity into selection strategies will be increasingly necessary to achieve sustainable production with changing atmospheric composition, while reductions in O 3 precursor emissions will likely benefit world food production and reduce atmospheric concentrations of an important greenhouse gas.

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Monensin was fed at 0 and 33 ppm in a crossover trial designed to determine the effect of this compound on ruminal and postruminal utilization of a corn-based diet and bacterial protein synthesis in abomasally fistulated steers. Monensin decreased (P greater than .10) ruminal true digestion of organic matter (OMc, corrected for bacterial cell synthesis) and apparent ruminal digestion of starch by 19%, but had not effect on apparent total tract digestion of OM or starch. Apparent ruminal and total tract digestibilities of crude protein (CP) were unchanged. Monensin decreased (P greater than .07) the contribution of bacterial N to total abomasal N (52 vs 58%), and increased (P greater than .06) the contribution of ruminally undegraded feed N (46 vs 40%), but had not effect on total N or amino acids recovered from the abomasum. Efficiency of bacterial protein synthesis (grams bacterial CP/100 g ruminally digested OMc) was unchanged. Monensin decreased (P greater than .05) the fraction of bacterial N to total N digested postruminally (42 vs 50%) and increased (P greater than .05) the contribution of ruminally undegraded feed N digested postruminally (58 vs 50%). Monensin caused a greater proportion of feed N and starch to be digested in the intestines than in the rumen (with possibly greater resultant metabolic efficiency), and this may account for some of the benefits obtained from feeding this compound with high grain diets.

Six wether lambs (31 kg) were randomly assigned to two treatments (three lambs/treatment): a high protein intake (HP; 21 g N/d) or a low protein intake (LP; 12 g N/d). Each lamb received 860 g/d dry matter (DM) of a pelleted diet (75% corn-soybean meal, 25% cottonseed hulls) offered hourly in 24 equal portions. Single injections of 15N-labelled compounds were made into the ruminal NH3-N and blood urea-N pools to measure the rate of flux through, and transfer of N between, these and the bacterial N pool. Total tract digestibilities of DM and N were lower (P less than .05) for the LP than the HP treatment. Abomasal flows of total, feed or bacterial N tended to be greater (P greater than .05) in lambs fed HP than LP. Lambs fed HP excreted more (P less than .01) urinary N, yet retained a greater (P less than .01) amount of N than lambs fed LP (6.2 vs 1.8 and 9.7 vs 4.1 g N/d, respectively). Pool size and production rate for both ruminal NH3-N and blood urea-N were greater (P less than .05) for the HP than LP treatment. Lambs consuming HP degraded more (P less than .05) blood urea-N in the gastro-intestinal tract (13.4 vs 6.9 g N/d); however, lambs fed LP degraded a greater (P less than .05) percentage of synthesized body urea-N (88.7 vs 71.8%). Ruminal NH3-N absorption was greater (P less than .01) for the HP than LP treatment (3.1 vs .5 g N/d). Although the percentage of bacterial N derived from ruminal NH3-N was similar (P greater than .05) between diets (51.1 vs 63.9), a greater (P less than .05) percentage of bacterial N was derived from blood urea-N in lambs fed LP than HP (77.1 vs 30.2%). Lambs fed LP incorporated a greater (P less than .10) amount of blood urea-N into bacterial N than lambs fed HP (5.5 vs 2.6 g N/d). These data are interpreted to suggest that blood urea-N may provide a substantial quantity of N for bacterial protein synthesis and, thus, may be an important source of protein in the deficient animal. In addition, urea recycling may play an important role in the recovery of ruminal NH3-N lost through absorption in animals fed a high level of protein.

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Monensin was fed at levels of 0 and 33 ppm in a series of digestion and metabolism trials to determine its effect on utilization of high grain diets and on ruminal parameters in yearling steers. Monensin had no effect (P greater than .05) on apparent digestibility of dry matter, gross energy or starch when fed with a 90% corn diet (10.5% crude protein, dry matter basis). Monensin in this corn-based diet tended to increase crude protein digestibility (63.4 vs 61.3%) and decrease ruminal ammonia concentration (2.5 vs 6.5 mg/100 ml) measured 3 hr postprandially (P greater than .05). In metabolism trials with a 76% sorghum grain diet (11.7% crude protein, dry matter basis), monensin improved apparent digestibility of crude protein (P less than .05) but not dry matter or gross energy (P greater than .05). Retention of nitrogen, expressed as a percentage of nitrogen intake, tended to improve (24 vs 20%) in response to monensin addition to the sorghum grain-based diet (P greater than .05); a similar trend was observed (P greater than .05) for nitrogen retention expressed as a percentage of nitrogen absorbed (41 vs 36%). Total ruminal volatile fatty acid (VFA) concentrations measured 3 hr postprandially were not altered by monensin fed with either diet. The proportion of acetic acid d-creased (P less than .05) and that of propionic acid increased (P less than .05) in response to monensin fed with the corn-based diet. Monensin did not appreciably alter proporations of VFA when fed with the sorghum grain-based diet. Results suggest that a possible improvement in N utilization may account for some of the benefits of feeding monensin with high grain diets.