Antimicrobial resistance is amongst the primary concern in the field of veterinary medicine worldwide. The research was conducted to evaluate the resistance of Escherichia coli due to inappropriate antibiotic treatment. Escherichia coli in this research was cultured in vitro using 5-ml Tryptone Soya Broth (TSB), which was mixed with an antibiotic, then was incubated at 37 0C for four days (stage I). Subsequently, a part of the culture was transferred into another 5-ml TSB and incubated at 37 0C for four days (stage II). This procedure was undertaken continuously for stage III, IV, V,and VI (until day 24). Antibiotics in this research was oxytetracycline with three various doses, which are lower dose (1 mg/ml), normal dose (2 mg/ml), and higher dose (8 mg/ml). The development of the antrimocobial resistance was evaluated every four days, using disk diffusion method and the data were analysed descriptively. The results showed that the normal and higher doses of oxytetracycline has the same rate (day 16) in causing E. coli resistant to oxytetracycline. Therefore, the treatment of oxytetracycline with a normal and higher doses continuously could accelerate the emergence of antimicrobial resistance in Escherichia coli.
Feline panleukopenia (FPL) adalah salah satu penyakit kucing mematikan yang menyebabkan diare dan leukopenia. Tujuan penelitian ini adalah melakukan diagnosis FPL berdasar total leukosit dan deteksi antigen feline parvovirus (FPV-Ag) pada kucing diare. Penelitian ini menggunakan sampel darah dan swab feses dari 21 kucing yang menunjukkan gejala diare. Total leukosit dihitung dari sampel darah dan adanya antigen FPV dideteksi dari swab feses menggunakan FPV-Ag-kit. Kesesuaian diagnosis FPL berdasar leukopenia dan reaksi positif uji FPV-Ag dibandingkan dengan menggunakan uji kesesuaian Cohen’s Kappa. Hasil penelitian menunjukkan bahwa 14 dari 21 ekor kucing (66,7%) mempunyai total leukosit dibawah normal dan 7 dari 21 ekor kucing (33,3%) mempunyai total leukosit normal. Uji FPV-Ag didapatkan hasil 11 dari 21 ekor kucing (52%) positif dan 10 dari 21 ekor kucing (48%) negatif. Diagnosis FPL berdasar leukopenia dan uji FPV-Ag mempunyai kesesuaian yang baik dengan nilai kesesuaian 0,71. Berdasar penelitian ini disimpulkan bahwa diagnosis FPL berdasar total leukosit dan FPV-Ag mempunyai kesesuaian yang baik, sehingga kedua uji dapat dipakai sebagai dasar diagnosis FPL.
Bovine ephemeral fever (atau 3-day sickness) adalah penyakit demam akut pada sapi dan kerbau yang disebabkan oleh Ephemerovirus dari keluarga rhabdoviridae dan ditularkan oleh vektor arthropoda. Penyakit tersebut biasa terjadi di daerah tropis dan subtropis. Dalam banyak kasus, dampak utamanya adalah pada penurunan produktivitas, produksi susu, kondisi tubuh, gangguan reproduksi, dan periode pemulihan yang lama pada beberapa hewan. Gejala klinis bervariasi pada setiap individu hewan, tetapi pada umumnya diawali dengan demam yang bersifat bifasik melanjut menjadi polifasik. Mortalitas biasanya rendah, namun, peningkatan kasus berakibat fatal telah dilaporkan dalam beberapa wabah akhir-akhir ini. Penyakit ini tersebar luas diberbagai daerah di Indonesia. Secara umum tidak menimbulkan kerugian ekonomi yang besar, asalkan segera mendapatkan pertolongan medis yang memadai sehingga tidak terjadi komplikasi dengan penyakit lain. Tulisan ini bertujuan untuk mengulas penyebab, metode diagnosa, dan terapi yang dapat dilakukan untuk menanggulangi kejadian BEF.
Abstract. Widiyono I, Rusmihayati, Purnamaningsih H, Sahara A. 2023. Zoonotic gastrointestinal nematodes in pet cats in Yogyakarta (Indonesia) and their susceptibility to anthelmintics. Biodiversitas 24: 3332-3337. Domestic cats (Felis catus Linnaeus, 1758), which live close to humans, can host some zoonotic nematodes. Therefore, a study on gastrointestinal nematodes in domestic cats and their anthelmintic resistance is essential for controlling and preventing zoonotic nematodiasis. Our goal was to investigate the diversity of gastrointestinal nematodes in pet cats in Yogyakarta and their susceptibility to anthelmintics. One hundred seventy-four (174) out of 215 cat patients (80.93%) presented at the animal clinic of the Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia, and suspected gastrointestinal parasite infections suffered from nematodiasis. Ninety-eight (98) animals suffering from moderate to severe gastrointestinal nematodiasis with Egg Per Gram feces (EPG) >300 were included into nematode identification, clinical analyses, and evaluation of nematode susceptibility to anthelmintics. The animals were divided in three treatment groups, namely pyrantel pamoate at the dose of 5 mg/kg BW orally on D0 (OP), subcutaneous injection of ivermectin at the dose of 200 µg/kg BW on day 0 (SCI), and no medication as a Control (C). Fecal samples were collected on day 0 and 10 for parasitological examinations. The results showed that pet cats were infected with Toxocara sp. and Ancylostoma sp., with the incidence of single infection by Toxocara sp., Ancylostoma sp., and mixed infection at 50%, 36.73%, and 13.27%, respectively. The animals were clinically healthy or showing clinical signs, e.g., anorexia, diarrhea, vomiting, and dull hair. Pyrantel pamoate and ivermectin showed >98% efficacy against these nematodes. In conclusion, there were two zoonotic gastrointestinal nematodes in pet cats in Yogyakarta, namely Ancylostoma and Toxocara, which still showed high susceptibility to pyrantel pamoate and ivermectin.
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