Background The purpose of this study was to analyze the association between the genetic polymorphism of genes ( PAX6 , SOSTDC1 and FAM20B ) and the susceptibility to mesiodens. Methods This study was carried out on 50 patients with mesiodens and 50 controls. The family history of each patient with mesiodens were recorded. Genomic DNA was extracted from saliva samples, and single nucleotide polymorphisms were detected in all exons and exon/intron boundaries of PAX6 , SOSTDC1 and FAM20B using Sanger sequencing. The data were analyzed using pearson chi-square test with theoretical frequency ≥ 5. For theoretical frequency less than 5 but at least 1 (≤20% cell), the data were analyzed by continuity correction. For the rest, Fisher’s Exact test was used. A P -value< 0.05 was considered statistically significant. The Odds ratio (OR) and confidence intervals (CI) were recorded. Results Three polymorphisms were detected in PAX6 . Two polymorphisms were detected in SOSTDC1 . Twenty-nine polymorphisms were detected in FAM20B . Although, the T allele of FAM20B (rs3766626) appears to be associated with mesiodens ( P = 0.051), there were no significant differences of PAX6 / SOSTDC1 / FAM20B gene polymorphisms between the two groups. The T allele of FAM20B (rs3766626) was associated with susceptibility to two mesiodens ( P < 0.001; OR = 8.333; CI = 2.516–27.600). Conclusions Lack of association between PAX6 / SOSTDC1 / FAM20B gene polymorphisms and mesiodens in the population studied was detected. Further studies with large samples on T allele of FAM20B (rs3766626) are needed. Electronic supplementary material The online version of this article (10.1186/s12903-019-0788-3) contains supplementary material, which is available to authorized users.
Purpose: To discuss effects of Hederin (Hed) to tooth movement process in rats. Materials and methods: 54 rats were divided into Model and Hed groups at 7 d, 14 d and 21 d, establishment of rat tooth movement model, the Hed and Model group injected Hed [5 mg/(kg· d)] and equal volume normal saline intraperitoneally respectively, Take the medicine regularly every night. After 14 days, 9 rats in each group were killed, BV/TV, Tb. SP and trabecular number (Tb. N) were measured by Mirco CT. Using TRAP staining and HE staining to observe osteoclasts number and pathology change. The relative protein expressions were measured by SP staining. Results: Compared with Model group, BV/TV and Tb.N were significantly down-regulation and Tb.Sp was significantly up-regulation in Hed group (P < 0.05, respectively); meanwhile, tooth movement and osteoclast number were significantly improved in Hed groups at 7 d, 14 d and 21 d (P <0.05, respectively). By SP staining, compared with Model group, ADRB2 and RANKL proteins expression were significantly enhanced at 7 d, 14 d and 21 d (P <0.05, respectively). Conclusion: Hed could promote alveolar bone resorption and increase the expression of ADRB2 and RANKL during orthodontic tooth movement.
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