Effects of Hederin (Hed) on Alveolar Bone Microstructure During Tooth Movement in Rats

Abstract

Purpose: To discuss effects of Hederin (Hed) to tooth movement process in rats. Materials and methods: 54 rats were divided into Model and Hed groups at 7 d, 14 d and 21 d, establishment of rat tooth movement model, the Hed and Model group injected Hed [5 mg/(kg· d)] and equal volume normal saline intraperitoneally respectively, Take the medicine regularly every night. After 14 days, 9 rats in each group were killed, BV/TV, Tb. SP and trabecular number (Tb. N) were measured by Mirco CT. Using TRAP staining and HE staining to observe osteoclasts number and pathology change. The relative protein expressions were measured by SP staining. Results: Compared with Model group, BV/TV and Tb.N were significantly down-regulation and Tb.Sp was significantly up-regulation in Hed group (P < 0.05, respectively); meanwhile, tooth movement and osteoclast number were significantly improved in Hed groups at 7 d, 14 d and 21 d (P <0.05, respectively). By SP staining, compared with Model group, ADRB2 and RANKL proteins expression were significantly enhanced at 7 d, 14 d and 21 d (P <0.05, respectively). Conclusion: Hed could promote alveolar bone resorption and increase the expression of ADRB2 and RANKL during orthodontic tooth movement.

Role of Topical and Systemic Medications in Orthodontics: A Review

“…The effects of a specific COX-2 inhibitor called "Rofecoxib" and a conventional NSAID called "Diclofenac" were examined in relation to the suppression of TMO in rats. The results showed that both drugs significantly impeded dental motion, though partially in the context of Rofecoxib and completely in the [12] Hederin (Hed) Alveolar bone resorption During TMO, Hed may enhance the expression of RANKL and ADRB2 and encourage alveolar bone resorption. Herniyati H et al 2023 [13] Robusta coffee extract (Coffea canephora)…”

“…They function by blocking prostaglandin synthesis and calcium absorption in the intestines, which in turn causes a rise in resorption of bone and a direct reduction of osteoblastic activity. The majority of animal investigations found that the chronic group requiring long-term corticosteroid medication had an increase in tooth movement rate [12][13][14][15][16][17][18][19][20]. On the other hand, investigations on acute corticosteroid consumption revealed decreased bone turnover.…”

“…It was discovered that clinically and economically, locally administered calcitriol increases TMO in humans in a dose-dependent manner. [12][13][14][15][16][17][18][19][20].…”

“…The biological sequence of processes described above may provide a succinct overview of current knowledge of intricate connections and processes taking place alveolar bone and PDL after mechanical stress or chemical mediator activity. These regulators' combined effects with mechanical forces might be inhibitory, preservative, or synergistic [11,12].…”