Toxoplasma gondii is a zoonotic agent of great importance in veterinary and public health. The aim of this study was to identify T. gondii by IHC (immunohistochemistry) in different sheep tissues and to determine if an association exists between the results obtained by this method and those obtained by the Modified Agglutination Test (MAT). Tissue specimens of twenty-six sheep seroreactive for T. gondii were selected for histopathological evaluation. The presence of T. gondii was investigated in brain, liver and heart samples by IHC and a possible anti-T. gondii antibody cross reactions with other parasites. McNemar's, Chi-square and Fisher's Exact Tests were applied for the statistical analysis of the results. The analysed tissues showed at least one of the following histopathological changes: mild-to-moderate congestion, focal polymorphonuclear inflammatory infiltrate and multifocal or focal mononuclear inflammatory infiltrate. Sarcocystis spp. were identified in the histological sections from both the heart and diaphragm tissues of 88.5% (23/26) of the animals. A total of 46.2% (12/26) of the T. gondii seroreactive sheep was also positive for T. gondii by IHC in at least one organ (brain, liver or heart). The liver IHC-positivity for T. gondii was statistically equivalent to the global individual IHC-positivity, according to McNemar's test. In addition, IHC allowed the detection of T. gondii in infected animals regardless of the titration observed in the MAT. The statistical difference observed between the three organs when comparing the low titration group, suggested that the heart might be the most suitable organ to detect T. gondii infection by IHC. The IHC results in this study revealed that almost half of MAT positive animals could serve as potential sources of infection for humans because bradyzoites were identified in different tissues, regardless of the MAT titration.
©2009 by Anais Brasileiros de DermatologiaA história da dermatologia moderna começa na Europa, em especial na França, entre os séculos XV e XVI, onde médicos começam a se interessar por problemas cutâneos. No Brasil, a dermatologia tem seu início em 1882, com a instalação do primeiro Serviço Clínico de Doenças da Pele na Policlínica Geral do Rio de Janeiro.A partir das descobertas da microbiologia, em finais do século XIX e início do século XX, e da dinâmi-ca induzida pelo ensino da dermatologia como especialidade, os estudos nesta área evoluíram até atingir seu atual nível de excelência.A dermatologia teve, nas últimas décadas, grande crescimento tanto quantitativo como qualitativo. O conhecimento dos mecanismos patogênicos das dermatoses foi muito ampliado, bem como se incorporaram novas terapêuticas que têm beneficiado de maneira crescente os portadores de dermatoses.
The importance of the Health Sciences Descriptors-DeCSfor the Anais Brasileiros de Dermatologia A importância dos descritores em Ciências da Saúde-DeCS para os Anais Brasileiros de Dermatologia Journals are the fastest mean to disseminate new investigations and knowledge in a certain field of study. Journals should be indexed, that is, described and identified by their contents in national and international reference databases, so that researchers will be respected by their peers, the results of their investigations be reported and information shared. The main databases related to health are the Science Citation Index/ISI, Medline, Lilacs and Embase (Excerpta Médica). The Anais Brasileiros de Dermatologia is indexed in the database Lilacs (Latin American and Caribbean Literature on Health Sciences) but its highest-priority goal is indexing in the database Medline, in order to assure international visibility, reporting and dissemination of its articles. It is worth mentioning that in order to achieve this goal, any scientific article submitted to approval by the peer-reviewers of Anais must comply with international standards, as required by international databases. Two standards could be mentioned: Vancouver, an international standardization on bibliographic reference, and the Health Sciences descriptors, usually known as DeCS, created by Bireme and based on the Medical Subject Headings-MeSH, from the U.S. National Library of Medicine. The DeCS is a structured vocabulary designed to enable the use of common terms for indexing and retrieval of scientific articles in the databases Medline and Lilacs.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.