2013
DOI: 10.1016/j.vetpar.2012.09.022
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Abstract: Toxoplasma gondii is a zoonotic agent of great importance in veterinary and public health. The aim of this study was to identify T. gondii by IHC (immunohistochemistry) in different sheep tissues and to determine if an association exists between the results obtained by this method and those obtained by the Modified Agglutination Test (MAT). Tissue specimens of twenty-six sheep seroreactive for T. gondii were selected for histopathological evaluation. The presence of T. gondii was investigated in brain, liver a… Show more

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Cited by 23 publications
(23 citation statements)
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“…The IHC assay performed in the present study was an important tool for the identification of T. gondii in tissues and, consequently, in the confirmation of the disease. The advantage of using IHC was also reported by Motta et al (2008) and Silva et al (2013a), who obtained similar results in sheep tissues. Although toxoplasmosis can be presumptively diagnosed through histopathology, as structures compatible with this protozoan can be visualized (ROSA et al, 2001;LANGONI, 2001), a definitive diagnosis can be obtained using IHC (ANDRADE et al, 2007;CASAGRANDE et al, 2013), which emphasizes the relevance of this test for diagnostic certification.…”
Section: Discussionsupporting
confidence: 80%
“…The IHC assay performed in the present study was an important tool for the identification of T. gondii in tissues and, consequently, in the confirmation of the disease. The advantage of using IHC was also reported by Motta et al (2008) and Silva et al (2013a), who obtained similar results in sheep tissues. Although toxoplasmosis can be presumptively diagnosed through histopathology, as structures compatible with this protozoan can be visualized (ROSA et al, 2001;LANGONI, 2001), a definitive diagnosis can be obtained using IHC (ANDRADE et al, 2007;CASAGRANDE et al, 2013), which emphasizes the relevance of this test for diagnostic certification.…”
Section: Discussionsupporting
confidence: 80%
“…This differed from result obtained by Silva (2011), among naturally infected sheep, who reported that the positive staining rate was 52% (12/25), using IHC. Among sheep in Rio de Janeiro, Brazil, Silva et al (2013) concluded that IHC was proven to be effective in making the definitive diagnosis of toxoplasmosis. This was not confirmed in the present study, because the agreement between the PCR and IHC results was moderate (k = 0.44).…”
Section: Discussionmentioning
confidence: 99%
“…Examination by histopathology is widely employed for the diagnosis of T. gondii with only few studies describing the immunohistochemical detection of the protozoan parasite (Pereira-Bueno et al, 2004). However, in a study to determine the association between serology (MAT) and presence of cysts in tissues (IHC) of infected animals, conclusion was drawn that MAT positive animals could serve as potential for human infection because bradyzoites were found in different tissues, regardless of the MAT titration (Silva et al, 2013). Zoonotic visceral leshmaniases for which the domestic dog represents the main reservoir host in urban environments is also one of the important protozoan diseases of humans (WHO, 2010;Chhabra & Singla, 2014 ).…”
Section: Immunohistochemistrymentioning
confidence: 99%