Background
Blumea eriantha DC is the Indian medicinal remedy, mainly distributed in the States of India. It possesses wide array of medicinal properties.
Aim
To execute green synthesis approach for the preparation of silver and iron nanoparticles by using alcoholic Blumea ErianthaDC extract and to verify the biological potential of the prepared nanoparticles as a hair growth promoter.
Patients/Method
Extract was mixed with silver nitrate and ferric chloride for synthesis of silver and iron nanoparticles, respectively. Prepared nanoparticles were confirmed by UV, FT‐IR, SEM, X‐ray diffraction, and TEM. The qualitative and quantitative analysis was performed namely hair growth initiation, hair growth completion, hair length, hair weight, histopathological studies, skin thickness, and length of the hair follicle.
Results
The prepared nanoparticles were observed as a blend of spherical and irregular shape with an average particle size of 35nm. The transition of anagen hair follicles was observed in approximately 63.43 % of animals treated with Minoxidil, whereas 2% and 5% Blumea erianthasilver nanoparticles treated animal group exhibited 33.02% and 60.93%, respectively. The animal groups treated with 2% and 5% iron nanoparticles of Blumea Eriantha showed 44.09 and 38.89% in anagen induction, respectively, which suggest the hair growth‐promoting potential of the extract of Blumea erianthaDC.
Conclusion
Thus, it can be concluded that silver nanoparticles of Blumea Eriantha exhibit promising hair growth promoting activity.
Anew simple, convenient and suitable spectrophotometric method for simultaneous determination of Furosemide and Spironolactone in combined dosage form has been developed and validated. Simultaneous equation method (Vierordt’s method) was used for determination of Furosemide and Spironolactone in combined dosage form. For spectrophotometric method development double distilled water and ethanol were used as a solvent in the ratio of (20:80). The proposed method was quantitatively evaluated in terms of linearity, precision, accuracy, lower limit of detection (LOD) and quantification (LOQ), recovery and robustness. All the parameters were found to be within the acceptance limit. λmax of Furosemide and Spironolactone was found to be 275 and 237 nm respectively. Beer’s law was obeyed over the concentration ranges of 2-10 μg mL−1 for both Furosemide and Spironolactone respectively. The % assay for commercial formulation was found to be 99.60%±0.0500 for Furosemide and 100.26%±1.17 for Spironolactone by the proposed methods. The overall recovery was observed to be 100.38±0.09% for Furosemide and 100.49±0.4197% for Spironolactone by simultaneous equation method (Vierordt’s method). LOD and LOQ were 0.76 and 2.32 μg mL−1 for Furosemide, 1.99 and 6.04 μg mL−1 for Spironolactone. A new simple, convenient, precise, rapid, accurate and economical and reliable spectrophotometric method was developed and validated for the analysis of Furosemide and Spironolactone in bulk drug and their formulations.
In this study, the synthesis of nanoparticles and their biological evaluation were carried out. A green synthetic approach synthesized silver nanoparticles (AHAgNPs) using an Artocarpus heterophyllus leaf extract. Parameter optimization was performed using Design Expert Ver. 13. The effects of variables like the concentration on the response, particle size, and entrapment efficiency of synthesized AHAgNPs were monitored via analysis of variance. The optimized AgNPs were characterized using ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy. Scanning electron microscopy and transmission electron microscopy were used to determine the size and shape of nanoparticles. In vitro, antioxidant and antimicrobial potential were determined using standard protocols. The optimized nanoparticles were spherical, with an average 100-110 nm particle diameter. The synthesized nanoparticles showed effective antioxidant, antibacterial, and antifungal activity. In addition, AHAgNPs showed increased biological activities.
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