In vivo and in vitro hair growth‐promoting effect of silver and iron nanoparticles synthesized via Blumea eriantha DC plant extract

Abstract

Background Blumea eriantha DC is the Indian medicinal remedy, mainly distributed in the States of India. It possesses wide array of medicinal properties. Aim To execute green synthesis approach for the preparation of silver and iron nanoparticles by using alcoholic Blumea ErianthaDC extract and to verify the biological potential of the prepared nanoparticles as a hair growth promoter. Patients/Method Extract was mixed with silver nitrate and ferric chloride for synthesis of silver and iron nanoparticles, respectively. Prepared nanoparticles were confirmed by UV, FT‐IR, SEM, X‐ray diffraction, and TEM. The qualitative and quantitative analysis was performed namely hair growth initiation, hair growth completion, hair length, hair weight, histopathological studies, skin thickness, and length of the hair follicle. Results The prepared nanoparticles were observed as a blend of spherical and irregular shape with an average particle size of 35nm. The transition of anagen hair follicles was observed in approximately 63.43 % of animals treated with Minoxidil, whereas 2% and 5% Blumea erianthasilver nanoparticles treated animal group exhibited 33.02% and 60.93%, respectively. The animal groups treated with 2% and 5% iron nanoparticles of Blumea Eriantha showed 44.09 and 38.89% in anagen induction, respectively, which suggest the hair growth‐promoting potential of the extract of Blumea erianthaDC. Conclusion Thus, it can be concluded that silver nanoparticles of Blumea Eriantha exhibit promising hair growth promoting activity.

Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study

“…Plant material of BE was stored from Bhinge-wadi, Atpadi region of Maharashtra, INDIA-415301, in the month of January from their natural territory. The taxonomical recognition of the BE was carried out with Herbaria (RMRC - 502) From Regional Centre of Indian Council of Medical Research, Nehru Nagar, Belgaum, KA-590010 [ 17 ]. In order to prevent degradation of the phyto-constituents, plant materials of BE were dried in oven at 40 ± 2 °C for a day and then stored in a air tight jar.…”

“…Plant extract should not have any irritating effect on the shaved region and should not cause skin lesions such as itchy eyes, skin rashes, inflammation, stuffy nose etc. [ 17 ]. Shaven dorsal region of the selected mice was properly cleaned with surgical spirit, further, BE alcoholic extracts (1.5% and 3%) and 3% Fraction A solution were rubbed on mice shaved region over 1 sq cm and the mice were monitored for 2 days for any skin lesions [ 19 ].…”

“…The dorsal skin biopsies of the mice from each group were taken and it was fixed in buffer formalin (10% neutral) solution. The sample applied mice tissues were fixed in paraffin wax and uniformly sliced (4 μm) and were stained with haematoxylin and eosin [ 17 ]. Digital photomicrographs were captured from respected area at fixed magnification of 100x [ 17 ].…”

“…The sample applied mice tissues were fixed in paraffin wax and uniformly sliced (4 μm) and were stained with haematoxylin and eosin [ 17 ]. Digital photomicrographs were captured from respected area at fixed magnification of 100x [ 17 ]. However, captured photo of mice tissues were cropped with width at 700 pixels.…”

“…In the A a yurvedic medicinal system, BE has been used in management of various diseases viz: studies on extracts of BE and their isolate(s) have shown anti-oxidant and anti-hyperlipidemic potential [ 11 ], anti-diarrhoeal activity [ 12 ], hypoglycemic activity [ 13 ], larvicidal activity [ 14 ], antimicrobial activity [ 15 ] and anti-cancer activity [ 16 ]. Moreover, the Bhinge et al (2020) have reported the hair growth potential of prepared iron and silver nanoparticles of BE extract [ 17 ]. Also, in India, BE has been claimed for hair growth promotion activity.…”