Abstract. A modification of Krijnen's cryopreservation method, using low intracellular concentrations of 17.5% glycerol and extracellular 4% sorbitol as additives, has been developed for liquid nitrogen freezing of small volumes of erythrocytes for a test cell panel. A technique for their subsequent successful storage in sterile, dextrose‐electrolyte maintenance solution (CP‐2) at 4°C after recovery is presented. By these procedures, the mean recovery of red cells after lytic losses from freezing and thawing injury was 94.1%. The manipulations involved in transferring, mixing and resuspending the red cells removed an additional 11.1–16.2%, resulting in an overall recovery of 80.5% for serologic tests. Less than 3% of the cells exhibited cumulative lysis at seven days maintenance in CP‐2 solution at 4°C, and the blood group antigens examined remained as potent as those in sterile, fresh ACD blood.
Abstract. A variant of blood group A, provisionally designated Ainn, that occurs in an overall frequency of approximately 1 in 6,000 in Finnish blood donors is described. Agglutination involving a very small number of erythrocytes was observable only microscopically, even with the most powerful immune anti‐A sera. However, potent anti‐A antibody eluates were produced from cells sensitized with both natural and immune anti‐A or anti‐A + B sera. Anti‐A1 agglutinins were demonstrated in the sera of all cases, and the saliva of secretors contained H but no A substances. Comparative examinations and previously published descriptions of other established variants clearly indicated that this was not identical with the Abantu, Am, Ax, or Ae1 phenotypes. These properties are similar to those reported for group Aend, except for enzyme pretreatment effecting macroscopically visible agglutination of A***inn cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.