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Introduction Regenerative endodontic protocols recommend White Mineral Trioxide Aggregate (WMTA) as a capping material due to its osteoinductive properties. Stem Cells from the Apical Papilla (SCAP) are presumed to be involved in this regenerative process, but the effects of WMTA on SCAP are largely unknown. Our hypothesis is that WMTA induces proliferation and migration of SCAP. Methods Here, we used an unsorted population of SCAP (passages 3 to 5) characterized by high CD24, CD146 and Stro-1 expression. The effect of WMTA on SCAP migration was assessed using transwells and its effect on proliferation was determined by the WST-1 assay. Fetal bovine serum (FBS) and calcium-chloride enriched media were used as positive controls. Results The SCAP analyzed here showed a low percentage of STRO-1+ and CD24+ cells. Both set and unset WMTA significantly increased the short-term migration of SCAP after 6 hours (P<0.05), whereas calcium-chloride enriched medium did after 24 hours of exposure. Set WMTA significantly increased proliferation on days 1 to 5 while calcium-enriched media showed a significant increase on day 7 with a significant reduction on proliferation afterwards. SCAP migration and proliferation were significantly and steadily induced by the presence of 2% and 10% FBS Conclusions Collectively, these data demonstrate that WMTA induced an early short-term migration and proliferation of a mixed population of stem cells from apical papilla as compared to a later and longer-term induction by calcium-chloride or FBS.

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This paper examined the impact of commercial thinning on selected wood properties of jack pine (Pinus banksiana Lamb.). Wood properties evaluated include wood density (ring density, earlywood ring density, and latewood ring density), percentage of latewood in the ring, and wood bending properties. Nonlinear, mixed-effect models have been developed using data from three commercially thinned sites in eastern Canada. Ring density followed the same pattern as percentage of latewood, in which cambial age, relative height, and ring width were found to have important effects. Earlywood and latewood ring densities changed within the juvenile wood zone until a plateau was reached. Ring width affected earlywood and latewood ring densities mainly in narrow rings. Wood bending stiffness (measured by modulus of elasticity) and strength (measured by modulus of rupture) increased with cambial age and wood density; whereas, wood strength was also affected by ring width. Commercial thinning did not influence the developed models, but it had an indirect effect through increased ring width.Résumé : Cette étude a examiné l'impact de l'éclaircie commerciale sur certaines propriétés du bois de pin gris (Pinus banksiana Lamb.). Ces propriétés incluaient la densité du bois (densité des cernes, densité du bois initial et densité du bois final), la proportion de bois final dans chaque cerne et les propriétés mécaniques en flexion. Des modèles non liné-aires mixtes ont été développés à partir de données provenant de trois stations qui avaient subi une éclaircie commerciale dans l'est du Canada. La densité des cernes s'est révélée être fortement influencée par l'âge cambial, la hauteur relative et la largeur des cernes, suivant ainsi un patron de variation similaire à celui de la proportion de bois final. La densité du bois initial et celle du bois final ont changé à l'intérieur de la zone de bois juvénile pour atteindre un plateau par la suite. La largeur des cernes a aussi affecté ces densités, en particulier dans le cas des cernes étroits. Les modules d'élasticité (MOE) et de rupture (MOR) en flexion ont augmenté avec l'âge cambial et la densité du bois. De plus, le module de rupture a été affecté par la largeur des cernes. L'éclaircie commerciale n'a pas changé les paramètres des modèles, mais elle a eu une influence indirecte à cause de son effet sur la largeur des cernes.[Traduit par la Rédaction]