Root fOLMS cecccccescceecccesceeesecccecnececececcecssseecesasesecenaeeccsteceseeeseecsnnneesnenneeenscenensseemseeneseonsecensesuencenneseans 24 Alig DOAUCS cecoccmcecccocacsacscccesecesssanessessseraesesnesesesenesessesecnsncosecconeatecsssoneeeaeensesseestsetsnsesseeeneee 24 Coriaria NOAULES ceccccccocccccccccesccsescecescecseseseecesesesnesescernecesecaneneuseeceeseaeeceetsesseteecesetsnscetensecsenene QO -Nodules on the Elaecagnaceae......ccscccsessccccecsessassccnssscesseceeesssnesecsssaesenesensssnecosssnecaneenasectneee ZO Ceanothus todtles cocccccccccccoscscco-ccsucsuvsvssossnessceneeneeneronssessesesecseceenetstencenseenesateeneecessesseseseesenee OD Meyrica nodules -.n..cessecescecssccesceceesceeeeeseneeseeceeteneatectecesenenssssenecneaseee menmeccenanaesnmecnsenenaeateaeass Podocar pus nOdules -...-.-sceccccccecececceccceceeeneeccereeeeneeseneecenssensecnmmemnessteneeesnsnesnenemamemenenaesenseatens Nodules on the Cycadaceae -...n..ssssssssssssscsnsssoseecosssesescessenesseneeseessenesesnenconesensessnsesscatecsatees COSUAVING TOAUICS ceccecccmcoccccsecccsscoescssesessssesceseeeesesoesteseeenecesesecotssssnenetestneneacoesesersesessesnesenenseee

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Various bacteria, actinomycetes, and filamentous fungi decomposed methionine, but only certain aerobic bacteria isolated from soil decomposed it in the absence of other organic substrates. These bacteria could grow on methionine as the only organic substrate and source of nitrogen and sulfur. Methionine was first deaminated and then demethiolated with production of methanethiol, part of which was oxidized to dimethyl disulfide. The amount of methanethiol that was oxidized varied with different cultures. A bacterial culture initially unable to grow on methionine developed capacity to do this in a medium which contained methionine and other growth substrates. The two sulfur products, methanethiol and dimethyl disulfide, are volatile and escaped from the media, resulting in a decrease in the sulfur content proportional to the amount of methionine decomposed.

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A unilateral ophthalmomyiasis posterior in a 5-year-old female spayed Domestic Long-haired cat of a third or fourth stage instar Cuterebra spp. larvae is reported. The cat was presented for depression and anorexia. The organism was found on physical examination at presentation. The cat was euthanized because of the worsening systemic condition. The larva was demonstrated by histopathology with coagulation necrosis and hemorrhage of the optic nerve, retina and choroid, and anterior uveitis. No significant cerebrum and anterior brain stem lesions were found.

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Soil fungi that attacked methionine required a utilizable source of energy such as glucose for growth. This is an example of co-dissimilation. Experiments with one of the fungi, representative of the group, are reported. In the absence of glucose, pregrown mycelium, even when depleted of energy reserves, oxidatively deaminated methionine with accumulation of a-keto-y-methyl mercapto butyric acid and ahydroxy-,y-methyl mercapto butyric acid. When glucose was provided, all of the sulfur of methionine was released as methanethiol, part of which was oxidized to dimethyl disulfide. No sulfate, sulfide, or hydrosulfide products were detected. Evidence was obtained that deaminase and demethiolase were constitutive. Deamination preceded demethiolation and a-keto butyric acid accumulated as a product of the two reactions. Other carbon residues were a-hydroxy butyric acid and a-amino butyric acid. Inability of the fungus to metabolize a-keto butyrate was responsible for its inability to utilize methionine as a source of carbon and energy. Several other fungi isolated from soil grew on a-amino butyrate but could not grow on methionine owing to inability to demethiolate it.