SUMMARYIsolates of bovine herpesvirus 1 (BHV-1) are associated with a variety of clinical manifestations. To determine if a single form of BHV-I was responsible for the different virus-associated diseases or whether subpopulations of various isolates produced different clinical symptoms, studies were initiated to examine the DNA restriction enzyme patterns and nucleic acid homology between virus isolates from respiratory infections and other clinical syndromes. Differences between the genomes of several virus isolates were detected using DNA restriction enzyme analyses. However, nucleic acid hybridization studies of the virus DNAs using filter and liquid hybridization indicated at least a 95~ genetic homology between the virus isolates from different types of infections. Additionally, these studies demonstrated that the DNA of BHV-1 had an average molecular weight of 84 x 106.Bovine herpesvirus 1 (BHV-1) has been associated with a variety of clinical syndromes. These are primarily respiratory, but may also inclfide genital infections, encephalitis, ocular carcinoma and abortion. The virus associated with respiratory diseases has been referred to as infectious bovine rhinotracheitis (IBR) virus, whereas the virus isolate associated with genital infections has been termed infectious pustular vulvovaginitis (IPV) virus (Kahrs, 1977;Pastoret et al., 1982). Misra et al. (1983) reported that isolates of BHV-I could be divided into three distinct groups based upon the virus DNA restriction enzyme patterns. As in the situation of herpes simplex virus types 1 and 2 in humans, the possibility exists that respiratory and genital infections may be caused by partially related viruses that appear serologically similar, but by analysis of the virus DNA represent different virus types. Consequently, the investigations reported here were initiated to determine the amount of genetic homology between a respiratory isolate of BHV-1 and other clinical isolates using restriction enzyme analysis and nucleic acid hybridization of virus DNA.Since reports indicated that DNA from BHV-1 isolates associated with respiratory infections (IBR virus) had different restriction patterns from the DNA isolated from viruses associated with genital infections (IPV virus) (Engels et al., 1981), we initially differentiated virus isolates using restriction enzyme analysis and nucleic acid hybridization. The BHV-1 isolates used in these studies were IBR virus Los Angeles (LA), a respiratory disease isolate, obtained from the American Type Culture Collection and an IPV virus isolate obtained from the Department of Microbiology, School of Veterinary Medicine, University of California, Davis. A variety of isolates from various clinical disease states were also obtained from the School of Veterinary Medicine at Washington State University, Pullman, Washington and from the Agriculture Experiment Station at the University of Nevada. Bovine embryonic lung cells were used for all experiments and cultured in Dulbecco's MEM with 5 ~ foetal calf serum. Virus DNA fo...
Six drugs were fed continuously to susceptible trout held in known Myxosotna cerebralis contaminated water in an attempt to prevent infection. Drug efficacy was judged by comparing numbers of spores which developed in medicated and non-medicated fish. Fish after having been fed furazolidone in two tests had fewer spores than the other groups.
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