Investigations of the incidence of bovine trichomoniasis in nevada and of the efficacy of immunizing cattle with vaccines containing

Kvasnicka, William G.; Taylor, Robert E. L.; Huang, Jiaoyan; Hanks, D.; Tronstad, R. J.; Bosomworth, A.; Hall, Mark R.

doi:10.1016/0093-691x(89)90479-2

Cited by 54 publications

(34 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…11,18 -20 The disease is highly prevalent in the US with 16-44% of tested herds in California and Nevada being infected. 21,22 Studies of immune responses showed IgG1 and IgA antibodies to T. foetus were present in reproductive secretions during and after infection. 23 Since this organism is an extracellular parasite, antibodies should be critical for protection.…”

Section: Introductionmentioning

confidence: 99%

Bovine Trichomoniasis as a Model for Development of Vaccines against Sexually‐transmitted Disease

Corbeil¹,

Munson

Campero³

et al. 2001

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Bovine Trichomoniasis as a Model for Development of Vaccines against Sexually‐transmitted Disease

Corbeil¹,

Munson

Campero³

et al. 2001

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

“…Because the disease is of considerable economic importance, there has been much interest in immunoprophylaxis and immunotherapy. This has resulted in a commercially available vaccine 20,21 and much information on protective immune responses. A major surface antigen, detected by monoclonal antibodies TF1.17 and TF1.15, 16 was found to contain a lipophosphoglycan (LPG) protein complex and to be released from the surface as soluble glycosylated antigen (SGA).…”

mentioning

confidence: 99%

Uterine Mast Cells and Immunoglobulin-E Antibody Responses During Clearance ofTritrichomonas foetus

Corbeil

Campero

Rhyan³

et al. 2005

Vet Pathol

View full text Add to dashboard Cite

Abstract. We showed earlier that Tritrichomonas foetus-specific bovine immunoglobulin (Ig)G1 and IgA antibodies in uterine and vaginal secretions are correlated with clearance of this sexually transmitted infection. Eosinophils have been noted in previous studies of bovine trichomoniasis but the role of mast cells and IgE responses have not been reported. The hypothesis that IgE and mast cell degranulation play a role in clearance was tested in 25 virgin heifers inseminated experimentally and infected intravaginally with T. foetus strain D1 at estrus and cultured weekly. Groups were euthanatized at 3, 6, 9, or 12 weeks, when tissues were fixed and secretions were collected for culture and antibody analysis. Immunohistochemistry using a monoclonal antibody to a soluble lipophosphoglycan (LPG)-containing surface antigen (TF1.17) demonstrated antigen uptake by uterine epithelial cells. Lymphoid nodules were detected below antigen-positive epithelium. Little IgG2 antibody was detected but IgG1, IgA, IgM, and IgE T. foetus-specific antibodies increased in uterine secretions at weeks 6 and 9 after infection. This was inversely proportional to subepithelial mast cells numbers and most animals cleared the infection by the sampling time after the lowest mast cell count. Furthermore, soluble antigen was found in uterine epithelium above inductive sites (lymphoid nodules). Cross-linking of IgE on mast cells by antigen and perhaps LPG triggering appears to have resulted in degranulation. Released cytokines may account for production of predominantly Th2 (IgG1 and IgE) and IgA antibody responses, which are related to clearance of the infection.

show abstract

“…Several studies have demonstrated various forms of increased resistance to infection with T. foetus resulting from parenteral immunization (3,8,13,15,16,18,19). One study observed an initial infection rate in cattle immunized with killed T. foetus that was equal to the infection rate in controls; however, the immunized animals cleared the infection more rapidly than the controls did (13).…”

Section: Discussionmentioning

confidence: 99%

Antibody Responses of Cattle Immunized with the Tf190 Adhesin of Tritrichomonas foetus

Voyich¹,

Ansotegui

Swenson

et al. 2001

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

The antibody response patterns of cattle after subcutaneous and intranasal immunizations with adhesin Tf190 of Tritrichomonas foetus were investigated. Reactions of antibody from cattle parenterally immunized with Tf190 revealed antigen specificity and Tf190 sensitization in the majority of the animals, as determined by Western blotting. The results also demonstrated strong preimmune immunoglobulin G2 (IgG2) binding to T. foetus antigens not seen in IgG1 profiles. Subcutaneous injections of Tf190 resulted in significant (P < 0.05) increases in serum IgG1 and IgG2 titers over time, as determined by parasite specific enzyme-linked immunosorbent assay. Immune sera also significantly inhibited parasite adhesion to mammalian cell lines compared to the level of inhibition obtained with preimmune sera (P < 0.05). Intranasal immunization with Tf190 failed to produce measurable parasite-specific antibody in serum; however, this immunization route did result in significant (P < 0.05) increases in parasite-specific IgA titers in cervical mucus secretions from immunized animals that were more resistant to intravaginal challenge with T. foetus than controls. These results suggest that systemic immunization with Tf190 results in serum antibody production and antiparasitic adhesin antibodies. Additionally, the results of challenge experiments with intranasally immunized animals suggests that Tf190 primes protective immune responses that lead to lower rates of infection among these animals.The sexually transmitted parasitic protozoan Tritrichomonas foetus, the causative agent of bovine trichomoniasis, results in fetal loss due to abortion and increased management costs due to unproductive cows. It is often found on the mucosal surfaces of the female reproductive tract or in the epithelial glands of the penis of the bull (20,21,23,28). There is no approved chemotherapy in the United States, and infected animals are usually culled to control disease in the herd (14). Infected animals can often self-cure, perhaps as a consequence of infection-induced acquired immunity; however, reinfection is common and infected bulls can carry the infection for life, serving as a reservoir for the disease and suggesting that immunity from natural infection rarely protects against challenge. Therefore, the reported herd incidence remains near 10%, despite the availability of a commercial vaccine (15). The present vaccine is also problematic because of its incomplete efficacy, since its use results in only an approximately 30% rate of reduction in the incidence of abortion (15).Advancements in chemotherapy and vaccine development are complicated by a general lack of knowledge of the immune effector functions that contribute to protection and insufficient understanding of the pathogenesis of trichomoniasis. Parasitespecific antibody responses in cattle to natural T. foetus infection (immunoglobulin G1 [IgG1], IgG2, and IgA isotypes) have been demonstrated by a variety of assays and with a variety of parasite antigens (3,9,15,17,25) and in experiment...

show abstract

Investigations of the incidence of bovine trichomoniasis in nevada and of the efficacy of immunizing cattle with vaccines containing

Cited by 54 publications

References 10 publications

Bovine Trichomoniasis as a Model for Development of Vaccines against Sexually‐transmitted Disease

Bovine Trichomoniasis as a Model for Development of Vaccines against Sexually‐transmitted Disease

Uterine Mast Cells and Immunoglobulin-E Antibody Responses During Clearance ofTritrichomonas foetus

Antibody Responses of Cattle Immunized with the Tf190 Adhesin of Tritrichomonas foetus

Contact Info

Product

Resources

About